Pathogenomic comparison of human extraintestinal and avian pathogenic Escherichia coli - Search for factors involved in host specificity or zoonotic potential

Autor: Jörg Hacker, Pierre Germon, Philippe Bauchart, Annie Brée, Eric Oswald, Ulrich Dobrindt
Přispěvatelé: University of Würzburg, Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, CHU Toulouse [Toulouse], German Academy of Sciences Leopoldina, Partenaires INRAE
Jazyk: angličtina
Rok vydání: 2010
Předmět:
animal structures
avian pathogenic E. coli pathogenomics
Virulence Factors
risque zoonotique
[SDV]Life Sciences [q-bio]
Virulence
Biology
medicine.disease_cause
Microbiology
03 medical and health sciences
chemistry.chemical_compound
Pathogenomics
Pathogenic Escherichia coli
transcriptome comparison host specificity
Escherichia coli
medicine
Animals
Humans
Gene
Escherichia coli Infections
Phylogeny
Poultry Diseases
030304 developmental biology
Genetics
Comparative Genomic Hybridization
0303 health sciences
Extraintestinal Pathogenic Escherichia coli
030306 microbiology
Escherichia coli Proteins
Gene Expression Profiling
Temperature
Sequence Analysis
DNA
zoonotic risk
biology.organism_classification
Bacterial adhesin
Infectious Diseases
chemistry
Extraintestinal pathogenic E. coli
Aerobactin
Chickens
Zdroj: Microbial Pathogenesis
Microbial Pathogenesis, Elsevier, 2010, 49 (3), pp.105-115. ⟨10.1016/j.micpath.2010.05.004⟩
ISSN: 0882-4010
1096-1208
Popis: International audience; Avian pathogenic Escherichia coli (APEC) and human extraintestinal pathogenic E. coli (ExPEC) cause various diseases in humans and animals and cannot be clearly distinguished by molecular epidemiology and genome content. We characterized traits of eight representative human ExPEC and APEC variants to either support the zoonotic potential or indicate factors involved in host specificity. These strains were very similar regarding phylogeny, virulence gene content and allelic variation of adhesins. Host- or serogroup-specific differences in type 1-, P-, S/F1C-fimbriae, curli, flagella, colicin and aerobactin expression or in vivo virulence were not found. Serogroup-dependent differences in genome content may depend on the phylogenetic background. To identify traits involved in host specificity, we performed transcriptome analysis of human ExPEC IHE3034 and APEC BEN374 in response to human (37 degrees C) or avian (41 degrees C) body temperature. Both isolates displayed similar transcriptional profiles at both temperatures. Transcript levels of motility/chemotaxis genes were repressed at 41 degrees C. The hdeAB and cadA genes involved in acid stress resistance, although often induced at 41 degrees C, could not be correlated with host specificity. Beside strain-specific effects, the common behavior of both strains at human or avian body temperature supports the idea of a potential zoonotic risk of certain human ExPEC and APEC variants.
Databáze: OpenAIRE
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