Anti-inflammatory effects of Morchella esculenta polysaccharide and its derivatives in fine particulate matter-treated NR8383 cells
| Autor: | Wan Li, Lin-Lin Liang, Hui-Yun Zhang, Yong-Ming Lu, Yu Liu, Yan Chen, Shomaila Mehmood, Zheng-Nan Cai |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Programmed cell death
Anti-Inflammatory Agents Apoptosis Inflammation 02 engineering and technology complex mixtures Biochemistry Cell Line Flow cytometry 03 medical and health sciences Ascomycota Structural Biology Macrophages Alveolar medicine Animals Molecular Biology 030304 developmental biology chemistry.chemical_classification 0303 health sciences Reactive oxygen species medicine.diagnostic_test Chemistry Spectrum Analysis NF-kappa B Fungal Polysaccharides General Medicine 021001 nanoscience & nanotechnology Molecular biology Rats IκBα Alveolar macrophage Particulate Matter Tumor necrosis factor alpha medicine.symptom Reactive Oxygen Species 0210 nano-technology Biomarkers |
| Zdroj: | International Journal of Biological Macromolecules. 129:904-915 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2019.02.088 |
| Popis: | Fine particulate matter (PM2.5) exposure could cause many acute and chronic respiratory diseases. In this study the protective effects of polysaccharide from Morchella esculenta (FMP-1) and its derivatives against PM2.5-induced inflammation were evaluated. By flow cytometry and ELISA analysis, sulfated polysaccharide SFMP-1 showed the best protective effect in reducing PM2.5-induced cell death, cell apoptosis and production of tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β), which was accompanied by a diminished level in reactive oxygen species (ROS) formation caused by PM2.5 in rat alveolar macrophage NR8383 cells. Furthermore, the mechanism was studied by immunofluorescence, qRT-PCR and western blotting. SFMP-1 could down-regulate the expression of inducible NO synthesis (iNOS) and cyclooxygenase-2 (COX-2) at both mRNA and protein levels in PM2.5-treated cells. The PM2.5-induced phosphorylation of nuclear factor-kappa B (NF-κB) was also reduced through suppressing nuclear translation of the NF-κB and inhibiting the degradation and phosphorylation of IκBα. These results indicated that SFMP-1 could protect NR8383 cells from PM2.5-induced inflammation by inhibiting NF-κB activation. |
| Databáze: | OpenAIRE |
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