Identification of 1-lysophosphatidylethanolamine (C16:1) as an antimicrobial compound in the housefly, Musca domestica
| Autor: | Elke Clynen, Liliane Schoofs, Arnold DeLoof, Karen Meylaers, Désiré Daloze |
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| Rok vydání: | 2004 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Antifungal Agents Bacillus thuringiensis Microbial Sensitivity Tests Saccharomyces cerevisiae medicine.disease_cause Biochemistry Houseflies Escherichia coli medicine Animals Housefly Nuclear Magnetic Resonance Biomolecular Molecular Biology Chromatography High Pressure Liquid Chromatography Molecular Structure biology Molecular mass biology.organism_classification Antimicrobial Yeast Anti-Bacterial Agents Insect Science Lysophospholipids Antibacterial activity Bacteria |
| Zdroj: | Insect Biochemistry and Molecular Biology. 34:43-49 |
| ISSN: | 0965-1748 |
| DOI: | 10.1016/j.ibmb.2003.09.001 |
| Popis: | We observed that a methanolic whole body extract of uninfected last instar larvae of the housefly, Musca domestica, displayed antifungal and antibacterial activity. We have further purified this extract to a single active fraction using reversed phase high performance liquid chromatography. The pure fraction inhibited growth of the Gram-positive bacteria Bacillus thuringiensis and the yeast Saccharomyces cerevisiae, but not the Gram-negative bacteria Escherichia coli. The active compound was determined to have a molecular mass of 451.2 Da. Further analysis by nuclear magnetic resonance identified the substance as mono-unsaturated 1-lysophosphatidylethanolamine (C(16:1)) (1-LPE). The structurally different and more common 2-LPE have been described as mediators of the antimicrobial activity of rimenophenazine antibiotic agents (Van Rensburg et al., 1992). Our results suggest that the isolated 1-LPE displays a higher activity in comparison, possibly based on structure-specific differences in activity. |
| Databáze: | OpenAIRE |
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