Genetic Dissection of Oligogenic Resistance to Bacterial Wilt in Tomato
| Autor: | Nevin D. Young, G E McGill, Sharon R. Aarons, D. Danesh |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Genetic Markers
Chromosome 7 (human) Genetics biology Genetic Linkage Physiology Bacterial wilt Chromosome Mapping food and beverages Chromosome Locus (genetics) General Medicine biology.organism_classification Immunity Innate Gene mapping Genetic marker Pseudomonas Vegetables Botany Genotype Allele Agronomy and Crop Science Crosses Genetic Polymorphism Restriction Fragment Length Plant Diseases |
| Zdroj: | Molecular Plant-Microbe Interactions. 7:464 |
| ISSN: | 0894-0282 |
| DOI: | 10.1094/mpmi-7-0464 |
| Popis: | To study resistance to bacterial wilt (caused by Pseudomonas solanacearum) in tomato, we analyzed 71 F2 individuals from a cross between a resistant and a susceptible parent with 79 DNA markers. F2 plants were inoculated by two methods: bacteria were injected into shoots of cuttings or poured into soil surrounding wounded roots. Disease responses were scored on a scale of 0 to 5. Statistical comparisons between DNA marker genotypes and disease phenotypes identified three genomic regions correlated with resistance. In plants inoculated through roots, genomic regions on chromosomes 6 and 10 were correlated with resistance. In plants inoculated through shoots, a region on chromosome 7 was significant, as were the regions on chromosomes 6 and 10. The relative impact of resistance loci on disease response differed between shoot and root inoculations. To confirm the existence of a partial resistance gene on chromosome 6, an F2 individual homozygous for the resistant parent's alleles on chromosomes 7 and 10, but heterozygous for markers on chromosome 6, was selfed. Analysis of the F3 progeny confirmed that a partial resistance locus was located on chromosome 6, very close to CT184. The presence of a partial resistance locus on chromosome 10 was similarly confirmed by analysis of progeny of another F2 plant chosen on the basis of its marker phenotype. |
| Databáze: | OpenAIRE |
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