Spatially resolved chemical analysis of cicada wings using laser-ablation electrospray ionization (LAESI) imaging mass spectrometry (IMS)
| Autor: | Jessica K. Román, Sungmin Hong, Catherine E. Dana, Callee M. Walsh, Kyoo D. Jo, Donald M. Cropek, Junho Oh, Marianne Alleyne, Nenad Miljkovic |
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| Rok vydání: | 2017 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Materials science Laser ablation electrospray ionization Electrospray ionization Analytical chemistry 02 engineering and technology Mass spectrometry 01 natural sciences Biochemistry Mass spectrometry imaging Analytical Chemistry Ion Matrix (chemical analysis) Hemiptera Ionization Animals Wings Animal Sample preparation Sulfones Amines 010401 analytical chemistry Esters Phosphorus Compounds 021001 nanoscience & nanotechnology Amides Lipids Hydrocarbons 0104 chemical sciences Laser Therapy 0210 nano-technology |
| Zdroj: | Analytical and bioanalytical chemistry. 410(7) |
| ISSN: | 1618-2650 |
| Popis: | Laser-ablation electrospray ionization (LAESI) imaging mass spectrometry (IMS) is an emerging bioanalytical tool for direct imaging and analysis of biological tissues. Performing ionization in an ambient environment, this technique requires little sample preparation and no additional matrix, and can be performed on natural, uneven surfaces. When combined with optical microscopy, the investigation of biological samples by LAESI allows for spatially resolved compositional analysis. We demonstrate here the applicability of LAESI-IMS for the chemical analysis of thin, desiccated biological samples, specifically Neotibicen pruinosus cicada wings. Positive-ion LAESI-IMS accurate ion-map data was acquired from several wing cells and superimposed onto optical images allowing for compositional comparisons across areas of the wing. Various putative chemical identifications were made indicating the presence of hydrocarbons, lipids/esters, amines/amides, and sulfonated/phosphorylated compounds. With the spatial resolution capability, surprising chemical distribution patterns were observed across the cicada wing, which may assist in correlating trends in surface properties with chemical distribution. Observed ions were either (1) equally dispersed across the wing, (2) more concentrated closer to the body of the insect (proximal end), or (3) more concentrated toward the tip of the wing (distal end). These findings demonstrate LAESI-IMS as a tool for the acquisition of spatially resolved chemical information from fragile, dried insect wings. This LAESI-IMS technique has important implications for the study of functional biomaterials, where understanding the correlation between chemical composition, physical structure, and biological function is critical. Graphical abstract Positive-ion laser-ablation electrospray ionization mass spectrometry coupled with optical imaging provides a powerful tool for the spatially resolved chemical analysis of cicada wings. |
| Databáze: | OpenAIRE |
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