Intracellular Position of Centrioles and the Direction of Homeostatic Epithelial Cell Movements in the Mouse Cornea
| Autor: | Takayuki Nagasaki, Erika L. Silverman, John C. Merriam, Jin Zhao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Histology Mouse Cornea Centriole Cell Biology Cornea 03 medical and health sciences Basal (phylogenetics) 0302 clinical medicine Cell Movement medicine Animals Homeostasis Centrioles Epithelial Cells Articles Epithelium eye diseases Cell biology Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure 030221 ophthalmology & optometry Female sense organs Anatomy Intracellular |
| Popis: | Corneal epithelial cells exhibit continuous centripetal movements at a rate of about 30 µm per day, but neither the driving force nor the mechanism that determines the direction of movements is known. To facilitate the investigation of homeostatic cell movement, we examined if the intracellular position of a centriole can be used as a directional marker of epithelial cell movements in the mouse cornea. A direction of cell movements was estimated in fixed specimens from a pattern of underlying subepithelial nerve fibers. Intracellular position of centrioles was determined by gamma-tubulin immunohistology and plotted in a narrow strip along the entire diameter of a cornea from limbus to limbus. When we determined the position of centrioles in the peripheral cornea where cell movements proceed generally along a radial path, about 55% of basal epithelial cells contained a centriole in the front half of a cell. However, in the central cornea where cells exhibit a spiral pattern of movements, centrioles were distributed randomly. These results suggest that centrioles tend to be positioned toward the direction of movement in corneal basal epithelial cells when they are moving centripetally at a steady rate. |
| Databáze: | OpenAIRE |
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