Bioinformatics analysis of gene expression profiles of dermatomyositis
| Autor: | Feng‑Hua Lan, Zhao‑Lei Cui, Ye Bai, Fan‑Cui Hua, Weng‑Jing Yang, Liang‑Yuan Chen |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cancer Research Paraneoplastic Syndromes Gene regulatory network Computational biology Biology Biochemistry Dermatomyositis Inflammatory myopathy Transcriptome 03 medical and health sciences 0302 clinical medicine Gene expression Genetics medicine Humans Gene Regulatory Networks Protein Interaction Maps KEGG Molecular Biology Gene Gene Expression Profiling Computational Biology medicine.disease Gene expression profiling Gene Ontology 030104 developmental biology Oncology Cancer research Molecular Medicine Signal transduction 030217 neurology & neurosurgery |
| Zdroj: | Molecular Medicine Reports. 14:3785-3790 |
| ISSN: | 1791-3004 1791-2997 |
| Popis: | Dermatomyositis (DM) is a type of autoimmune inflammatory myopathy, which primarily affects the skin and muscle. The underlying mechanisms of DM remain poorly understood. The present study aimed to explore gene expression profile alterations, investigate the underlying mechanisms, and identify novel targets for DM. The GSE48280 dataset, which includes data from five DM and five normal muscle tissue samples, was obtained from the Gene Expression Omnibus. Firstly, differentially expressed genes (DEGs) were screened by limma package in R. Subsequently, functional and pathway enrichment analyses were performed using ClueGO from Cytoscape. Finally, protein‑protein interaction (PPI) networks were constructed using STRING and Cytoscape, in order to identify hub genes. As a result, 180 upregulated and 21 downregulated genes were identified in the DM samples. The Gene Ontology enrichment analysis revealed that the type I interferon (IFN) signaling pathway was the most significantly enriched term within the DEGs. The Kyoto Encyclopedia of Genes and Genomes pathway analysis identified 27 significant pathways, the majority of which can be divided into the infectious diseases and immune system categories. Following construction of PPI networks, 24 hub genes were selected, all of which were associated with the type I IFN signaling pathway in DM. The findings of the present study indicated that type I IFNs may have a central role in the induction of DM. In addition, other DEGs, including chemokine (C‑C motif) ligand 5, C‑X‑C motif chemokine 10, Toll‑like receptor 3, DEXD/H‑Box helicase 58, interferon induced with helicase C domain 1, interferon‑stimulated gene 15 and MX dynamin‑like GTPase 1, may be potential targets for DM diagnosis and treatment. |
| Databáze: | OpenAIRE |
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