Combining mesenchymal stem cell sheets with platelet-rich plasma gel/calcium phosphate particles: a novel strategy to promote bone regeneration
| Autor: | Junhua Li, Zhongli Shi, Zhongming Huang, Tengfei Zhao, Yiying Qi, Gang Feng, Lie Niu, Tuoyu Di |
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| Rok vydání: | 2015 |
| Předmět: |
Calcium Phosphates
Male Bone Regeneration Mesenchymal stem cell sheet Osteocalcin Medicine (miscellaneous) chemistry.chemical_element Gene Expression Biocompatible Materials Calcium Mesenchymal Stem Cell Transplantation Biochemistry Genetics and Molecular Biology (miscellaneous) Rats Sprague-Dawley chemistry.chemical_compound Tissue engineering Platelet-rich plasma Osteogenesis Osteogenic differentiation Materials Testing Animals Femur Bone regeneration Cell Proliferation Calcium phosphate particles biology Tissue Engineering Tissue Scaffolds Research Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Cell Biology Molecular biology Tenomodulin Rats Calcein chemistry Immunology biology.protein Molecular Medicine Collagen |
| Zdroj: | Stem Cell Research & Therapy |
| ISSN: | 1757-6512 |
| Popis: | Background Promotion of bone regeneration is important for successful repair of bony defects. This study aimed to investigate whether combining bone marrow-derived mesenchymal stem cell (BMSC) sheets with platelet-rich plasma (PRP) gel/calcium phosphate particles could promote bone formation in the femoral bone defects of rats. Methods The proliferation and differentiation of BMSCs or BMSC sheets cultured with calcium phosphate particles and/or PRP were investigated in in vitro. In vivo, 36 2.5 × 5 mm bone defects were randomly divided into groups and treated with either BMSCs/PRP gel, calcium phosphate particles, PRP gel/calcium phosphate particles, a BMSC sheet/calcium phosphate particles, a BMSC sheet/PRP gel/calcium phosphate particles, or were left untreated (n = 6/group). A further 15 bone defects were treated with chloromethyl-benzamidodialkylcarbocyanine (CM-Dil)-labelled BMSC sheet/PRP gel/calcium phosphate particles and observed using a small animal in vivo fluorescence imaging system to trace the implanted BMSCs at 1 day, 3 days, 7 days, 2 weeks, and 4 weeks after surgery. Results The expression of collagen type I and osteocalcin genes of BMSCs or BMSC sheets treated with PRP and calcium phosphate particles was significantly higher than that of BMSCs or BMSC sheets treated with calcium phosphate particles or the controls (P P Conclusions Incorporation of an BMSC sheet into the PRP gel/calcium phosphate particles greatly promoted bone regeneration. These BMSC sheet and tissue engineering strategies offer therapeutic opportunities for promoting bone defect repair clinically. |
| Databáze: | OpenAIRE |
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