Comparison of the genetic variability of Blastocystis subtypes between human carriers from two contrasting climatic regions of México
| Autor: | María Durazo, Pablo Maravilla, Guadalupe Erendira Orozco-Mosqueda, Guiehdani Villalobos, Fernando Martínez-Hernández, Alejandro Urrea-Quezada, Isaac Villegas-Gómez, Jesús Hernández, Olivia Valenzuela, Mariana González-Díaz |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
Male 0301 basic medicine Microbiology (medical) Adolescent Genotype Climate 030231 tropical medicine Blastocystis Infections Biology Microbiology Gene flow Nucleotide diversity Young Adult 03 medical and health sciences 0302 clinical medicine Genetics Humans Genetic variability Child Mexico Molecular Biology Ribosomal DNA Phylogeny Ecology Evolution Behavior and Systematics Aged Genetic diversity Blastocystis Polymorphism Genetic Phylogenetic tree Haplotype Genetic Variation Infant Sequence Analysis DNA Middle Aged biology.organism_classification 030104 developmental biology Infectious Diseases Haplotypes RNA Ribosomal Child Preschool Carrier State Female |
| Zdroj: | Infection, Genetics and Evolution. 44:334-340 |
| ISSN: | 1567-1348 |
| DOI: | 10.1016/j.meegid.2016.07.036 |
| Popis: | Blastocystis sp. is an anaerobic intestinal microorganism commonly identified in the feces of several animals, including humans. Blastocystis exhibits high genetic polymorphism and at least 17 subtypes (ST) have been identified; ST1-ST3 are frequently found in the Americas. Furthermore, in vitro assays have shown that temperature and humidity can affect the viability of Blastocystis cysts. In this study, we describe the genetic variability and genetic differentiation among and within Blastocystis STs in adults and children from the cities of Hermosillo and Morelia cities, which represent arid and humid subtropical climatic regions of México, respectively. Phylogenetic and genetic diversity was assessed by analyzing a region of the small subunit ribosomal DNA (SSU rDNA) gene as a marker. Blastocystis ST3 and ST1 were associated with children from Hermosillo and Morelia, respectively. An analysis of the nucleotide diversity (π) and haplotype polymorphism (θ) indexes showed that they were similar within each ST, but different between ST1 and ST3. Interestingly, the group of symptomatic carriers from Hermosillo showed scarce mean nucleotide diversity compared to the asymptomatic carriers (0.0039±0.0030 and 0.0329±0.0286, respectively). Furthermore, the gene flow and genetic differentiation indexes between the children and adults suggested that the Blastocystis haplotypes in the adult carriers were "highly mobile" among humans, while the haplotypes found in the children were more isolated and genetically differentiated between them. |
| Databáze: | OpenAIRE |
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