Persistent ventricle partitioning in the adult zebrafish heart
| Autor: | Hannah R. Moran, Anastasia Felker, Catherine Pfefferli, Christian Mosimann, Anna Jaźwińska |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
lcsh:Diseases of the circulatory (Cardiovascular) system cardiac ventricle Biology Article 03 medical and health sciences lineage tracing 0302 clinical medicine medicine Pharmacology (medical) General Pharmacology Toxicology and Pharmaceutics Atrium (heart) Zebrafish 030304 developmental biology 0303 health sciences Heart development Lateral plate mesoderm Embryogenesis Cardiac Ventricle first heart field heart development Compartmentalization (fire protection) zebrafish biology.organism_classification Cell biology 030104 developmental biology medicine.anatomical_structure lcsh:RC666-701 Ventricle Embryonic Ventricle 030217 neurology & neurosurgery |
| Zdroj: | Journal of Cardiovascular Development and Disease Volume 8 Issue 4 Journal of Cardiovascular Development and Disease, Vol 8, Iss 41, p 41 (2021) |
| Popis: | The vertebrate heart integrates cells from the early-differentiating first heart field (FHF) and the later-differentiating second heart field (SHF) emerging from the lateral plate mesoderm. In mammals, this process forms the basis for the development of the left and right ventricle chambers and subsequent chamber septation. The single ventricle-forming zebrafish heart also integrates FHF and SHF lineages during embryogenesis, yet the contributions of these two myocardial lineages to the adult zebrafish heart remain incompletely understood. Here, we characterize the myocardial labeling of FHF descendants in both the developing and adult zebrafish ventricle. Expanding previous findings, late gastrulation-stage labeling usingdrl-driven CreERT2 recombinase with a myocardium-specific,myl7-controlledloxPreporter results in predominant labeling of FHF-derived outer curvature and the right side of the embryonic ventricle. Raised to adulthood, such lineage-labeled hearts retain broad areas of FHF cardiomyocytes in a region of the ventricle that is positioned at the opposite side to the atrium and encompasses the apex. Our data add to the increasing evidence for a persisting cell-based compartmentalization of the adult zebrafish ventricle even in the absence of any physical boundary. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|