Tumorigenic transformation of murine keratinocytes by the E5 genes of bovine papillomavirus type 1 and human papillomavirus type 16
| Autor: | Daniel DiMaio, David J. Riese, C Leptak, S. Ramón y Cajal, B H Horwitz, G P Dotto, R Kulke |
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| Jazyk: | angličtina |
| Rok vydání: | 1991 |
| Předmět: |
Keratinocytes
Genes Viral viruses Immunology Genetic Vectors Gene Expression Mice Nude Microbiology 3T3 cells Cell Line Mice Retrovirus Virology medicine Animals Humans Papillomaviridae Gene Bovine papillomavirus Bovine papillomavirus 1 Expression vector biology integumentary system Bovine Papillomavirus-1 virus diseases 3T3 Cells biology.organism_classification Molecular biology Tumor Virus Infections medicine.anatomical_structure Cell Transformation Neoplastic Cell culture Insect Science Cattle Neoplasm Transplantation Research Article |
| Zdroj: | Scopus-Elsevier ResearcherID |
| Popis: | To examine the biological properties of the bovine papillomavirus type 1 (BPV) and human papillomavirus type 16 (HPV16) E5 genes, each was cloned separately into a retroviral expression vector and helper-free recombinant viruses were generated in packaging cell lines. The BPV E5 retroviruses efficiently caused morphologic and tumorigenic transformation of cultured lines of murine fibroblasts, whereas the HPV16 E5 viruses were inactive in these assays. In contrast, infection of the p117 established line of murine epidermal keratinocytes with either the BPV or the HPV16 E5 retrovirus resulted in the generation of tumorigenic cells. Pam212 murine keratinocytes were also transformed to tumorigenicity by the HPV16 E5 gene but not by the gene carrying a frameshift mutation. These results establish that the HPV16 E5 gene is a transforming gene in cells related to its normal host epithelial cells. |
| Databáze: | OpenAIRE |
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