Insights of Phenolic Pathway in Fruits: Transcriptional and Metabolic Profiling in Apricot (Prunus armeniaca)
| Autor: | Helena Gomez-Martinez, María Luisa Badenes, Francisco Gil-Muñoz, Almudena Bermejo, Elena Zuriaga |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Antioxidant DFR medicine.medical_treatment fruits 01 natural sciences Q04 Food composition lcsh:Chemistry Transcription (biology) Flavonol synthase lcsh:QH301-705.5 Spectroscopy Plant Proteins chemistry.chemical_classification biology Phenylpropanoid Phenolic pathway food and beverages General Medicine Apricots F60 Plant physiology and biochemistry Prunus armeniaca Computer Science Applications Biochemistry Metabolome Prunus Oxidoreductases FLS phenolic pathway Catalysis Article Fruits Inorganic Chemistry 03 medical and health sciences Dihydroflavonol-4-reductase (DFR) medicine Physical and Theoretical Chemistry Molecular Biology Gene Phenylalanine Ammonia-Lyase Flavonoids Organic Chemistry Polyphenols Q01 Food science and technology biology.organism_classification Phenyalanine ammonia-lyase (PAL) Alcohol Oxidoreductases 030104 developmental biology Enzyme Genes chemistry lcsh:Biology (General) lcsh:QD1-999 Polyphenol PAL biology.protein Transcriptome F30 Plant genetics and breeding 010606 plant biology & botany |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 3411, p 3411 (2021) International Journal of Molecular Sciences electronico ReDivia. Repositorio Digital del Instituto Valenciano de Investigaciones Agrarias instname Volume 22 Issue 7 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | There is an increasing interest in polyphenols, plant secondary metabolites, in terms of fruit quality and diet, mainly due to their antioxidant effect. However, the identification of key gene enzymes and their roles in the phenylpropanoid pathway in temperate fruits species remains uncertain. Apricot (Prunus armeniaca) is a Mediterranean fruit with high diversity and fruit quality properties, being an excellent source of polyphenol compounds. For a better understanding of the phenolic pathway in these fruits, we selected a set of accessions with genetic-based differences in phenolic compounds accumulation. HPLC analysis of the main phenolic compounds and transcriptional analysis of the genes involved in key steps of the polyphenol network were carried out. Phenylalanine ammonia-lyase (PAL), dihydroflavonol-4-reductase (DFR) and flavonol synthase (FLS) were the key enzymes selected. Orthologous of the genes involved in transcription of these enzymes were identified in apricot: ParPAL1, ParPAL2, ParDFR, ParFLS1 and ParFLS2. Transcriptional data of the genes involved in those critical points and their relationships with the polyphenol compounds were analyzed. Higher expression of ParDFR and ParPAL2 has been associated with red-blushed accessions. Differences in expression between paralogues could be related to the presence of a BOXCOREDCPAL cis-acting element related to the genes involved in anthocyanin synthesis ParFLS2, ParDFR and ParPAL2. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|