Real-time monitoring of 3T3-L1 preadipocyte differentiation using a commercially available electric cell-substrate impedance sensor system
| Autor: | Carminita L. Frost, Adrienne L. Edkins, Julia Joos-Vandewalle, Earl Prinsloo, Adam H. Kramer |
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| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
Cell Cytological Techniques Biophysics Biosensing Techniques Biology Biochemistry Dexamethasone Rosiglitazone Mice Computer Systems Internal medicine 1-Methyl-3-isobutylxanthine 3T3-L1 Cells medicine Adipocytes Electric Impedance Animals Insulin Real time analysis Molecular Biology End point Adipogenesis Cell substrate Impedance sensor 3T3-L1 Cell Differentiation Cell Biology Cell biology Endocrinology medicine.anatomical_structure Thiazolidinediones Single plate |
| Zdroj: | Biochemical and biophysical research communications. 443(4) |
| ISSN: | 1090-2104 |
| Popis: | Real-time analysis offers multiple benefits over traditional end point assays. Here, we present a method of monitoring the optimisation of the growth and differentiation of murine 3T3-L1 preadipocytes to adipocytes using the commercially available ACEA xCELLigence Real-Time Cell Analyser Single Plate (RTCA SP) system. Our findings indicate that the ACEA xCELLigence RTCA SP can reproducibly monitor the primary morphological changes in pre- and post-confluent 3T3-L1 fibroblasts induced to differentiate using insulin, dexamethasone, 3-isobutyl-1-methylxanthine and rosiglitazone; and may be a viable primary method of screening compounds for adipogenic factors. |
| Databáze: | OpenAIRE |
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