Total Synthesis and Conformational Analysis of Naturally Occurring Lipovelutibols along with Lead Optimization of Lipovelutibol D

Autor:	Anil Kumar, Deepika Singh, Anup Singh Pathania, Ram A. Vishwakarma, Fayaz Ahmed Malik, Sonia Sharma, Varun Pratap Singh
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	chemistry.chemical_classification medicine.diagnostic_test Hydrogen bond Chemistry Stereochemistry General Chemical Engineering Total synthesis General Chemistry Aldehyde Article Trichoderma velutinum Intramolecular force Spectrophotometry medicine Cytotoxic T cell Cytotoxicity QD1-999
Zdroj:	ACS Omega ACS Omega, Vol 6, Iss 9, Pp 6070-6080 (2021)
ISSN:	2470-1343
Popis:	Four lipopeptaibols, namely, lipovelutibols A-D, were recently isolated from psychrotrophic fungus Trichoderma velutinum and reported to have significant cytotoxic activity against HL-60, MDA-MD-231, A549, and LS180 cancer cell lines. In the present study, these peptides were synthesized in a solution using a segment condensation approach. The conformational analysis of these peptides carried out using CD spectrophotometry revealed the formation of 310-helix, and the NMR-VT experiments showed intramolecular hydrogen bonding for NH-5, NH-6, and NH-7. Lipovelutibol D showed potent cytotoxic activity and was chosen for lead optimization. It involved N- and C-terminal truncation, N- and C-terminal modification, random deletion, l/d configuration replacement, and other synthetic analogues. These were tested against various breast cancer cell lines. The C-terminal aldehyde analogue resulting from lead optimization of lipovelutibol D was found to have almost twofold enhanced cytotoxicity against MDA-MB-231 breast cancer cell lines.
Databáze:	OpenAIRE
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