Dimerizable detergents as gene transfer vectors
| Autor: | Jean-Serge Remy, Jean-Paul Behr, Emmanuel Dauty, Thomas Blessing |
|---|---|
| Přispěvatelé: | Laboratoire de Chimie des Systèmes Fonctionnels, Centre National de la Recherche Scientifique (CNRS), Conception et application de molécules bioactives (CAMB), Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) |
| Předmět: |
education.field_of_study
Liposome 010405 organic chemistry Chemistry Population Dispersity Cationic polymerization Pharmaceutical Science Transfection [SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences 010402 general chemistry 01 natural sciences 0104 chemical sciences chemistry.chemical_compound Biochemistry Amphiphile Biophysics Molecule education DNA |
| Zdroj: | ResearcherID Journal of Liposome Research Journal of Liposome Research, Informa Healthcare, 2000, 10 (4), pp.321-327. ⟨10.3109/08982100009031102⟩ |
| ISSN: | 0898-2104 1532-2394 |
| Popis: | International audience; Cationic lipids are efficient vectors for DNA delivery in vitro. However, they condense DNA into large polymorphic particles, which severely limits their in vivo performances due to size-restricted diffusion. In contrast, detergents are capable of collapsing DNA into smaller particles but do not mediate cell transfection per se. We have succeeded in retaining the interesting features of both types of amphiphiles in a two-step process leading to monomolecular DNA particles stable in physiologic medium. Anionic DNA molecules were first individually condensed with a designed cationic cysteine-based detergent. The resulting small particles were then stabilised by spontaneous thiol dimerization of the cysteine-detergent into a cystine-lipid on the template DNA. Laser light scattering as well as electron microscopy revealed a monodisperse population of spherical particles that were stable for days in physiological conditions. With an appropriate choice of hydrocarbon chain length, monomolecular complexes exhibiting a typical lipid/DNA internal structure could be obtained. Their in vitro cell transfection properties compare favourably with those of Lipofectamine and PEI. |
| Databáze: | OpenAIRE |
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