Recombinant human type II phospholipase A2 lacks edema producing activity in rat
| Autor: | Celia M. Anderson, Douglas W. Morgan, Kenneth P. Meyers |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Male
Naja Venom Pharmacology complex mixtures Phospholipases A law.invention Phospholipase A2 stomatognathic system law Edema Crotalid Venoms Synovial Fluid medicine Animals Humans Synovial fluid Elapid Venoms Inflammation Phospholipase A biology respiratory system equipment and supplies biology.organism_classification Arthritis Experimental Recombinant Proteins Rats Phospholipases A2 Rats Inbred Lew Snake venom Immunology Recombinant DNA biology.protein lipids (amino acids peptides and proteins) medicine.symptom |
| Zdroj: | European Journal of Pharmacology. 235:45-50 |
| ISSN: | 0014-2999 |
| Popis: | The rat paw edema-inducing, acute inflammatory activity of four snake venom phospholipase A 2 s (PLA 2 ) ( Naja naja, Naja mocambique mocambique, Crotalus atrox and recombinant Naja naja naja ) and of recombinant human type II PLA 2 (rh-PLA 2 ) found in rheumatoid synovial fluid, were compared after a bolus subplantar injection. The snake venom-derived PLA 2 s, including the recombinant Naja naja naja , were potent inducers of paw edema. On the other hand, when given in similar amounts (protein and/or enzymatic activity), the rh-PLA 2 did not produce paw edema. Furthermore, the addition of Naja naja PLA 2 , blood plasma from rats with adjuvant arthritis or the E. coli -based enzymatic incubation mixture used to measure PLA 2 activity to the injection mixture containing rh-PLA 2 , did not result in paw edema-inducing activity. These results suggest that the lack of paw edema-inducing activity of rh-PLA 2 , may be due to significant structural differences between snake venom PLA 2 s and human PLA 2 which allow snake venom PLA 2 s, but not the human II PLA 2 , to express inflammatory activity as measured by paw edema in rat induced by a bolus injection. |
| Databáze: | OpenAIRE |
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