A Caenorhabditis elegans mass spectrometric resource for neuropeptidomics

Autor: Liliane Schoofs, Kurt Boonen, Isabel Beets, Sven Zels, Sven Van Bael, Liesbet Temmerman
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Proteomics
Technology
CONNECTOME
0302 clinical medicine
Structural Biology
Tandem Mass Spectrometry
Peptidomics
Caenorhabditis elegans
Spectroscopy
PRECURSORS
NEUROMODULATION
biology
Chemistry
Chemistry
Physical

BIOINFORMATICS
CLEAVAGE SITES
Physical Sciences
FMRFamide-like peptide
Neurohormones
Life Sciences & Biomedicine
Cell signaling
Proteases
Biochemistry & Molecular Biology
In silico
FLP
Neuropeptide
Computational biology
NLP
Biochemical Research Methods
03 medical and health sciences
ENDOGENOUS PEPTIDES
Animals
Amino Acid Sequence
LC-MS/MS
Caenorhabditis elegans Proteins
Biology
Science & Technology
Mass spectrometry
IDENTIFICATION
Neuropeptides
Chemistry
Analytical

biology.organism_classification
GENE
030104 developmental biology
030217 neurology & neurosurgery
Function (biology)
Chromatography
Liquid
Zdroj: Journal of the American Society for Mass Spectrometry
ISSN: 1044-0305
Popis: Neuropeptides are important signaling molecules used by nervous systems to mediate and fine-tune neuronal communication. They can function as neurotransmitters or neuromodulators in neural circuits, or they can be released as neurohormones to target distant cells and tissues. Neuropeptides are typically cleaved from larger precursor proteins by the action of proteases and can be the subject of post-translational modifications. The short, mature neuropeptide sequences often entail the only evolutionarily reasonably conserved regions in these precursor proteins. Therefore, it is particularly challenging to predict all putative bioactive peptides through in silico mining of neuropeptide precursor sequences. Peptidomics is an approach that allows de novo characterization of peptides extracted from body fluids, cells, tissues, organs, or whole-body preparations. Mass spectrometry, often combined with on-line liquid chromatography, is a hallmark technique used in peptidomics research. Here, we used an acidified methanol extraction procedure and a quadrupole-Orbitrap LC-MS/MS pipeline to analyze the neuropeptidome of Caenorhabditis elegans. We identified an unprecedented number of 203 mature neuropeptides from C. elegans whole-body extracts, including 35 peptides from known, hypothetical, as well as from completely novel neuropeptide precursor proteins that have not been predicted in silico. This set of biochemically verified peptide sequences provides the most elaborate C. elegans reference neurpeptidome so far. To exploit this resource to the fullest, we make our in-house database of known and predicted neuropeptides available to the community as a valuable resource. We are providing these collective data to help the community progress, amongst others, by supporting future differential and/or functional studies. ispartof: Journal of the American Society for Mass Spectrometry vol:29 issue:5 pages:879-889 ispartof: location:FL, Clearwater Beach status: published
Databáze: OpenAIRE