Bombyx mori Rad23 (BmRad23) contributes to the repair of UV-damaged BmNPV
| Autor: | Fangying Chen, Peng Wu, Lipeng Qiu, Keping Chen, Qi Tang, Xinyu Qi, Huiqin Chen, Zhaoyang Hu, Guohui Li |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Ultraviolet Rays DNA damage Health Toxicology and Mutagenesis Host-Cell Reactivation 01 natural sciences law.invention 03 medical and health sciences law Bombyx mori Animals Gene Reporter gene biology Chemistry fungi General Medicine Bombyx biology.organism_classification Molecular biology Nucleopolyhedroviruses Reverse transcriptase 010602 entomology 030104 developmental biology Recombinant DNA Baculoviridae Agronomy and Crop Science DNA Damage Nucleotide excision repair |
| Zdroj: | Pesticide Biochemistry and Physiology. 164:91-99 |
| ISSN: | 0048-3575 |
| DOI: | 10.1016/j.pestbp.2019.12.013 |
| Popis: | Baculoviruses have been developed as long-term and environmentally friendly biopesticides. However, solar ultraviolet radiation can reduce the activity of baculovirus. Radiation sensitive 23 (Rad23) can recognize DNA damage and is involved with nucleotide excision repair (NER). In the current study, BmRad23 was accumulated mainly within the nucleus. Host cell reactivation (HCR) assays have shown that BmRad23 significantly facilitated the expression of UV-damaged mCherry reporter gene. Reverse transcription quantitative PCR (RT-qPCR) result showed that the mRNA expression level of BmRad23 was increased in (Bombyx mori nuclear polyhedrovirus, BmNPV) BmNPV-infected BmN cells. However, the expression of BmRad23 was increased significantly when BmNPV budded viruses (BVs) or BmN cells were irradiated with UV light. Overexpression of BmRad23 promoted the mRNA levels of two UV-induced DNA damage repair genes which were from Bombyx mori and BmNPV, respectively. Meanwhile, the overexpression of BmRad23 in BmN cells was conducive to the proliferation of BmNPV and UV-damaged BmNPV. The recombinant BmNPV BVs expressing BmRad23 showed stronger resistance to UV radiation than the control virus. In conclusion, the results revealed that BmRad23 contributed to the proliferation of BmNPV and the repair of UV-damaged BmNPV, which would provide a reference for the development of efficient baculovirus pesticides against UV radiation. |
| Databáze: | OpenAIRE |
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