Calpains are activated by photodynamic therapy but do not contribute to apoptotic tumor cell death
| Autor: | Edgar R. Gomes, Carlos B. Duarte, Ramiro D. Almeida, Arsélio P. Carvalho |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Cancer Research
Indoles Time Factors Cell Survival medicine.medical_treatment Calpains Apoptosis Photodynamic therapy chemistry.chemical_compound Enzyme activator Cell Line Tumor Organometallic Compounds medicine Humans Caspase Photosensitizing Agents biology Calpain Singlet oxygen Lymphoblast Cancer medicine.disease Cell biology Enzyme Activation Photochemotherapy Oncology chemistry Caspases biology.protein |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| ISSN: | 0304-3835 |
| Popis: | Photodynamic therapy (PDT) of cancer is a promising technique based on the formation of singlet oxygen following irradiation of a sensitizer with visible light. In the present work we investigated the role of calpains in PDT, using the human lymphoblastoid CCRF-CEM cells and bisulfonated aluminum phthalocyanine (AlPcS2) as a sensitizer. Photosensitization induced apoptotic cell death and a time-dependent activation of calpains, as determined using the fluorogenic substrate succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin (SLLVY-AMC). However, inhibition of calpains with calpain inhibitor II or with PD 150606 did not affect the demise process. The results indicate that although calpains are activated in PDT, they do not play a major role in tumor cell death. http://www.sciencedirect.com/science/article/B6T54-4D7K70T-1/1/6ab9c47e98e82c782e8b8eebda86004d |
| Databáze: | OpenAIRE |
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