The Ccr4-Not Complex and yTAF1 (yTafII130p/yTafII145p) Show Physical and Functional Interactions
| Autor: | Laurent Maillet, Cécile Deluen, Martine A. Collart, Grégory Theiler, Marc Lemaire, Nicole Paquet, Nicole James, Miguel Molinete |
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| Přispěvatelé: | Centre médical universitaire de Genève (CMU), MAILLET, LAURENT |
| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
Cell Cycle Proteins
[SDV.GEN] Life Sciences [q-bio]/Genetics Transcription Factors TFII 0302 clinical medicine Transcription (biology) Gene Expression Regulation Fungal ddc:616 Repressor Proteins/genetics/metabolism Genetics 0303 health sciences Temperature Protein Binding/physiology Ribonucleases/metabolism DNA-Binding Proteins TAF1 Cell Cycle Proteins/chemistry/metabolism Protein Binding Transcription Factors/chemistry/genetics/metabolism Saccharomyces cerevisiae Proteins Macromolecular Substances Recombinant Fusion Proteins Ubiquitin-Protein Ligases Repressor Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins/genetics/metabolism Biology DNA-binding protein Fungal Proteins 03 medical and health sciences Ribonucleases Gene Expression Regulation Fungal/physiology Fungal Proteins/genetics/metabolism Molecular Biology Transcription factor 030304 developmental biology Transcriptional Regulation [SDV.GEN]Life Sciences [q-bio]/Genetics TATA-Binding Protein Associated Factors Recombinant Fusion Proteins/chemistry/genetics/metabolism Transcription Factors TFII/chemistry/metabolism Promoter Cell Biology Protein Structure Tertiary Repressor Proteins DNA-Binding Proteins/genetics/metabolism Protein Structure Tertiary/physiology Transcription Factor TFIID biology.protein Mutagenesis Site-Directed TATA-binding protein 030217 neurology & neurosurgery Transcription Factors |
| Zdroj: | Molecular and Cellular Biology Molecular and Cellular Biology, American Society for Microbiology, 2002, 22, pp.6735-6749. ⟨10.1128/MCB.22.19.6735-6749.2002⟩ Molecular and Cellular Biology, Vol. 22, No 19 (2002) pp. 6735-49 |
| ISSN: | 0270-7306 1098-5549 |
| DOI: | 10.1128/MCB.22.19.6735-6749.2002⟩ |
| Popis: | International audience; The Saccharomyces cerevisiae Ccr4-Not complex is a global regulator of transcription that is thought to regulate TATA binding protein (TBP) function at certain promoters specifically. In this paper, we show interactions between the essential domain of Not1p, which interacts with Not4p and Not5p, and the N-terminal domain of yTAF1. We isolated a temperature-sensitive nonsense allele of TAF1, taf1-4, which is synthetically lethal at the permissive temperature when combined with not4 and not5 mutants and which produces high levels of a C-terminally truncated yTAF1 derivative. Overexpression of C-terminally truncated yTAF1 is toxic in not4 or not5 mutants, whereas overexpression of full-length yTAF1 suppresses not4. Furthermore, mutations in the autoinhibitory N-terminal TAND domain of yTAF1 suppress not5, and the overexpression of similar mutants does not suppress not4. We find that, like Not5p, yTAF1 acts as a repressor of stress response element-dependent transcription. Finally, we have evidence for stress-regulated occupancy of promoter DNA by Not5p and for Not5p-dependent regulation of yTAF1 association with promoter DNA. Taken together with our finding that Not1p copurifies with glutathione S-transferase-yTaf1 in large complexes, these results provide the first molecular evidence that the Ccr4-Not complex might interact with yTAF1 to regulate its association at promoters, a function that might in turn regulate the autoinhibitory N-terminal domain of yTAF1. |
| Databáze: | OpenAIRE |
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