Site-directed psoralen crosslinking of DNA
| Autor: | Merrill Goldenberg, Charles R. Cantor, Wilma A. Saffran |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Organomercury Compounds
Stereochemistry medicine.medical_treatment chemistry.chemical_compound Plasmid Furocoumarins Methods medicine Trioxsalen heterocyclic compounds Sulfhydryl Compounds Psoralen Multidisciplinary Chemistry DNA DNA Restriction Enzymes Cross-Linking Reagents PBR322 Microscopy Electron Restriction site Biochemistry BamHI Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 79:4594-4598 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.79.15.4594 |
| Popis: | A technique has been developed for placing site-specific crosslinks into DNA by using a psoralen derivative containing a sulfhydryl group. Plasmid pBR322 was nicked at the unique BamHI restriction site, and mercurated pyrimidines were introduced by nick-translation. Then the psoralen was specifically directed to this site in the dark through a Hg-S linkage prior to irradiation to form a crosslink. The location of interstrand cross-links was examined by electron microscopy of Pst I-cut molecules and found to be at or near the BamHI site. |
| Databáze: | OpenAIRE |
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