Association of RNA Helicase A with Human Immunodeficiency Virus Type 1 Particles
| Autor: | Xiaofeng Guo, Chen Liang, Rodney S. Russell, Fei Guo, Bibhuti Bhusan Roy, Lawrence Kleiman, Jing Hu |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
DNA
Complementary Gene Products gag RNA-dependent RNA polymerase Biology Response Elements Autoantigens Biochemistry Cell Line DEAD-box RNA Helicases Transcription (biology) Gene expression Humans RNA Small Interfering Molecular Biology RNA Double-Stranded Viral Reverse Transcription RNA Cell Biology RNA Helicase A Virology Long terminal repeat Reverse transcriptase Neoplasm Proteins Protein Structure Tertiary HIV-1 Spliceosomes Dimerization RNA Helicases Plasmids |
| Zdroj: | Journal of Biological Chemistry. 281:12625-12635 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m510596200 |
| Popis: | RNA helicase A (RHA) belongs to the DEAH family of proteins that are capable of unwinding double-stranded RNA structure. In addition to its involvement in the metabolism of cellular RNA, RHA has been shown to stimulate RNA transcription from the long terminal repeat promoter of human immunodeficiency virus type 1 (HIV-1) as well as to enhance Rev/Rev response element-mediated gene expression. In this study, we provide evidence that RHA associates with HIV-1 Gag in an RNA-dependent manner. This interaction results in specific incorporation of RHA into HIV-1 particles. Knockdown of endogenous RHA in virus producer cells leads to generation of HIV-1 particles that are less infectious in part as a result of restricted reverse transcription. Therefore, RHA represents the first example of cellular RNA helicases that participate in HIV-1 particle production and promote viral reverse transcription. |
| Databáze: | OpenAIRE |
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