CircRFX3 contributes to glioma progression through the circRFX3-miR-1179/miR-1229-VASP axis
| Autor: | Li Li, Hongli Li, Yiwei Zhang, Huiqin Song |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Cancer Research
medicine.disease_cause Flow cytometry Glioma miR-1229 Genetics medicine RC254-282 Gene knockdown Reporter gene medicine.diagnostic_test QH573-671 Chemistry Cell growth Vasodilator-stimulated phosphoprotein Neoplasms. Tumors. Oncology. Including cancer and carcinogens Cell migration VASP medicine.disease Oncology Cancer research circRFX3 Carcinogenesis Primary Research miR-1179 Cytology |
| Zdroj: | Cancer Cell International, Vol 21, Iss 1, Pp 1-13 (2021) Cancer Cell International |
| ISSN: | 1475-2867 |
| Popis: | Background Circular RNAs (circRNAs) are implicated in the carcinogenesis of human cancers. However, the functional roles of circRFX3 in glioma are not elucidated. Methods Quantitative real-time polymerase chain reaction (qRT-PCR) assay was performed for the levels of circRFX3, RFX3, miR-1179, miR-1229 and vasodilator stimulated phosphoprotein (VASP). Actinomycin D assay and RNase R assay were employed to analyze the characteristics of circRFX3. Cell Counting Kit-8 (CCK-8) assay and colony formation assay were conducted for cell proliferation. Transwell assay was used for cell migration and invasion. Flow cytometry analysis was adopted for cell apoptosis. RNA pull-down assay, dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay were employed to analyze the interaction between miR-1179/miR-1229 and circRFX3 or VASP. Western blot assay was conducted for VASP protein level. Murine xenograft model assay was used to investigate the role of circRFX3 in vivo. Results CircRFX3 level was increased in glioma tissues and cells. Knockdown of circRFX3 suppressed glioma cell proliferation, migration and invasion and promoted apoptosis in vitro and repressed tumorigenesis of glioma in vivo. MiR-1179 and miR-1229 were identified to be the targets of circRFX3. MiR-1179 or miR-1229 inhibition reversed the impacts of circRFX3 knockdown on glioma cell malignant behaviors. Additionally, VASP was demonstrated to be the target gene of miR-1179 and miR-1229, and VASP overexpression abolished the effect of circRFX3 knockdown on glioma cell progression. Conclusion CircRFX3 served as a tumor promoter in glioma via modulating miR-1179/miR-1229-VASP axis, which might provide a novel target for glioma therapy. |
| Databáze: | OpenAIRE |
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