Detection of gyrA mutations associated with ciprofloxacin resistance in Neisseria gonorrhoeae by rapid and reliable pre-programmed short DNA sequencing
| Autor: | Baback Gharizadeh, Pål Nyrén, Nader Pourmand, Magnus Unemo, Michael S. Akhras, Bengt Wretlind |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Microbiology (medical)
Sexually transmitted disease DNA Mutational Analysis Drug resistance Biology medicine.disease_cause Article DNA sequencing Gonorrhea Anti-Infective Agents Bacterial Proteins Species Specificity Ciprofloxacin Drug Resistance Bacterial Genotype medicine Humans Point Mutation Pharmacology (medical) Codon Antibacterial agent Genetics Point mutation General Medicine Virology Neisseria gonorrhoeae Infectious Diseases DNA Gyrase Pyrosequencing |
| Zdroj: | International Journal of Antimicrobial Agents. 26:486-490 |
| ISSN: | 0924-8579 |
| DOI: | 10.1016/j.ijantimicag.2005.08.017 |
| Popis: | Quinolone resistance is rapidly increasing in Neisseria gonorrhoeae and is posing a significant public health threat that requires constant surveillance. A rapid and reliable mutation detection assay has been developed. The assay is based on pre-programmed short DNA sequencing and is designed to detect point mutations in the gyrA gene that are highly related to ciprofloxacin resistance, i.e. in codons 91 and 95. By developing an assay based on pyrosequencing and exploiting the pre-programmed nucleotide dispensation capability of this technology, the sequence comprising the mutations will be analysed and promptly reveal whether the N. gonorrhoeae pathogen carries resistance to ciprofloxacin. A panel of 40 N. gonorrhoeae clinical isolates, of which 27 phenotypically displayed decreased susceptibility or resistance to ciprofloxacin, was used in the present study. All point mutations in the short stretch of the N. gonorrhoeae gyrA gene were easily discriminated, and the genotypic results obtained by pre-programmed sequencing were mainly in agreement with the phenotypically identified decreased susceptibility or resistance to ciprofloxacin. The new method used in the present study has the potential for rapid and reliable identification of known as well as previously unknown drug resistance mutations. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect