Antimicrobial activities and matrix-assisted laser desorption/ionization mass spectrometry of Bacillus isolates from the marine sponge Aplysina aerophoba
| Autor: | Peter Franke, Jürgen Hofemeister, Gerhard Bringmann, Jörg Hacker, Christopher Wilde, Christian T. Pabel, Ute Hentschel, Barbara Adler, Joachim Vater |
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| Rok vydání: | 2002 |
| Předmět: |
biology
Base Sequence Lipoproteins Molecular Sequence Data Bacillus Sequence Analysis DNA biology.organism_classification Antimicrobial Isolation (microbiology) Mass spectrometry Applied Microbiology and Biotechnology DNA Ribosomal Microbiology Porifera Agar plate Matrix-assisted laser desorption/ionization Bacillus isolates Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Animals Biological Assay France Bacteria Phylogeny DNA Primers |
| Zdroj: | Marine biotechnology (New York, N.Y.). 5(5) |
| ISSN: | 1436-2228 |
| Popis: | The aim of this study was to isolate bacteria that are resistant to the strong antimicrobial metabolites characteristic of Aplysina aerophoba. For this purpose, bacterial isolation was performed on agar plates to which sponge tissue extract had been added. Following screening for antifungal and antimicrobial activities, 5 strains were chosen for more detailed analyses. 16S ribosomal DNA sequencing revealed that all isolates belonged to the genus Bacillus, specifically B. subtilis and B. pumilus. Using a combination of matrix-assisted laser desorption/ ionization mass spectrometry typing of whole cells and antimicrobial bioassays against selected reference strains, the bioactive metabolites were identified as lipopeptides. |
| Databáze: | OpenAIRE |
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