Mir106b-25 and Mir17-92 Are Crucially Involved in the Development of Experimental Neuroinflammation
| Autor: | Alessandra Mandelli, Annamaria Finardi, Paolo Dellabona, Martina Diceglie, Luca Carbone, Maya Fedeli, Roberto Furlan, Caterina Arnò, Giulia Casorati, Giuseppe De Santis |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
experimental autoimmune encephalomyelitis miR17-92 miR106b-25 Biology multiple sclerosis lcsh:RC346-429 Pathogenesis 03 medical and health sciences 0302 clinical medicine Immune system Downregulation and upregulation microRNA medicine Neuroinflammation lcsh:Neurology. Diseases of the nervous system Original Research Multiple sclerosis Experimental autoimmune encephalomyelitis medicine.disease MicroRNAs IL-17 030104 developmental biology Neurology Immunology Neurology (clinical) Interleukin 17 Th17 030217 neurology & neurosurgery |
| Zdroj: | Frontiers in Neurology, Vol 11 (2020) Frontiers in Neurology |
| ISSN: | 1664-2295 |
| Popis: | MicroRNAs (miRNAs) are single-stranded RNA that have key roles in the development of the immune system and are involved in the pathogenesis of various autoimmune diseases. We previously demonstrated that two members of the miR106b-25 cluster and the miR17-92 paralog cluster were upregulated in T regulatory cells from multiple sclerosis (MS) patients. The aim of the present work was to clarify the impact of miR106b-25 and miR17-92 clusters in MS pathogenesis. Here, we show that the mice lacking miR17-92 specifically in CD4+ T cells or both total miR106b-25 and miR17-92 in CD4+ T cells (double knockout) are protected from Experimental Autoimmune Encephalomyelitis (EAE) development while depletion of miR106b-25 only does not influence EAE susceptibility. We suggest that the absence of miR106b does not protect mice because of a mechanism of compensation of miR17-92 clusters. Moreover, the decrease of neuroinflammation was found to be associated with a significant downregulation of pro-inflammatory cytokines (GM-CSF, IFNγ, and IL-17) in the spinal cord of double knockout EAE mice and a reduction of Th17 inflammatory cells. These results elucidate the effect of miR106b-25 and miR17-92 deletion in MS pathogenesis and suggest that their targeted inhibition may have therapeutic effect on disease course. |
| Databáze: | OpenAIRE |
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