Control of renal sympathetic nerve activity by neurotransmitters in the spinal cord in Goldblatt hypertension
| Autor: | Alex Y.S. Sato, Ruy R. Campos, Maycon I.O. Milanez, Erika E. Nishi, Henrique de Azevedo Futuro Neto, Cássia T. Bergamaschi |
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| Rok vydání: | 2018 |
| Předmět: |
Male
medicine.medical_specialty Angiotensin receptor Sympathetic Nervous System Blood Pressure Kainate receptor 030204 cardiovascular system & hematology Baroreflex Kidney Kynurenic Acid Losartan Receptor Angiotensin Type 1 Renovascular hypertension 03 medical and health sciences 0302 clinical medicine Heart Rate Internal medicine medicine Animals Excitatory Amino Acid Agents Rats Wistar Molecular Biology Neurotransmitter Agents Angiotensin II receptor type 1 business.industry General Neuroscience medicine.disease Spinal cord Angiotensin II Rats Hypertension Renovascular Endocrinology medicine.anatomical_structure Spinal Cord Neurology (clinical) business 030217 neurology & neurosurgery Developmental Biology Ionotropic effect |
| Zdroj: | Brain Research. 1698:43-53 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/j.brainres.2018.06.025 |
| Popis: | The role of spinal cord neurons in renal sympathoexcitation remains unclear in renovascular hypertension, represented by the 2-kidney, 1-clip (2K1C) model. Thus, we aimed to assess the influence of spinal glutamatergic and AT1 angiotensin II receptors on renal sympathetic nerve activity (rSNA) in 2K1C Wistar rats. Hypertension was induced by clipping the renal artery with a silver clip. After six weeks, a catheter (PE-10) was inserted into the subarachnoid space and advanced to the T10-11 vertebral level in urethane-anaesthetized rats. The effects of intrathecally (i.t.) injected kynurenic acid (KYN) or losartan (Los) on blood pressure (BP) and rSNA were analysed over 2 consecutive hours. KYN induced a significantly larger drop in rSNA among 2K1C rats than among control (CTL) rats (CTL vs. 2K1C: −8 ± 3 vs. −52 ± 9 spikes/s after 120′). Los also evoked a significantly larger drop in rSNA among 2K1C rats than among CTL rats starting at 80′ after administration (CTL vs. 2K1C – 80 min: −10 ± 2 vs. –32 ± 6∗; 100 min: −15 ± 4 vs. −37 ± 9∗; 120 min: −12 ± 5 vs. −37 ± 8∗ spikes/s). KYN decreased BP similarly in the CTL and 2K1C groups; however, Los significantly decreased BP in the 2K1C group only. We found upregulation of AT1 gene expression in the T11-12 spinal segments in the 2K1C group but no change in gene expression for AT2 or ionotropic glutamate (NMDA, kainate and AMPA) receptors. Thus, our data show that spinal ionotropic glutamatergic and AT1 receptors contribute to increased rSNA in the 2K1C model, leading to the maintenance of hypertension; however, the participation of spinal AT1 receptors seems to be especially important in the establishment of sympathoexcitation in this model. The origins of those projections, i.e., the brain areas involved in establishing the activity of spinal glutamatergic and angiotensinergic pathways, remain unclear. |
| Databáze: | OpenAIRE |
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