Interactions of Hydrogen Peroxide with Interleukin-6 and Platelet-Derived Growth Factor in Determining Mesangial Cell Growth: Effect of Repeated Oxidant Stress
| Autor: | Richard C. Strange, H. M. Phillips, G. M. Aber, Peter W. Jones, R. J. D'souza |
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| Rok vydání: | 1993 |
| Předmět: |
Male
medicine.medical_specialty Time Factors Platelet-derived growth factor medicine.medical_treatment Rats Inbred WKY chemistry.chemical_compound Internal medicine medicine Animals Hydrogen peroxide Interleukin 6 Incubation Cells Cultured Platelet-Derived Growth Factor chemistry.chemical_classification Reactive oxygen species Dose-Response Relationship Drug biology Mesangial cell Interleukin-6 Growth factor Drug Synergism Hydrogen Peroxide General Medicine Glomerular Mesangium Rats Endocrinology chemistry Cell culture biology.protein Cell Division |
| Zdroj: | Clinical Science. 85:747-751 |
| ISSN: | 1470-8736 0143-5221 |
| DOI: | 10.1042/cs0850747 |
| Popis: | 1. This study examined the influence of H2O2, interleukin-6 and platelet-derived growth factor on the proliferation of rat mesangial cells. Mesangial cells were exposed to either a single pulse or three daily pulses of H2O2 (10−8-10−4 mol/l), alone or in combination with interleukin-6 (5 ng/ml) and/or platelet-derived growth factor (10 ng/ml). Proliferation was assessed after 24 h and 72 h of incubation using [3H]thymidine incorporation and cell counts. 2. Although one pulse of H2O2 had no significant effect on mesangial cell proliferation, three daily pulses of 10−6 mol/l H2O2 resulted in a significant increase in [3H]thymidine incorporation of 31 (52.6, 10.3)% (median and 75th-25th interquartile range) (P 3. These findings suggest that reactive oxygen species may play a major role in determining the mesangial cell proliferation that occurs in certain forms of glomerulonephritis, acting either alone or in combination with other growth factors. |
| Databáze: | OpenAIRE |
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