Identification of barley powdery mildew resistances in gene bank accessions and the use of gene diversity for verifying seed purity and authenticity
| Autor: | Marta Zavřelová, Antonín Dreiseitl |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Leaves Plant Science Plant Genetics 01 natural sciences Gene bank Disease Resistance Multidisciplinary biology Plant Anatomy Plant Fungal Pathogens Eukaryota food and beverages Agriculture Plants Spring Horticulture Germination Seeds Medicine Seasons Powdery mildew Research Article Genotype Science Plant Pathogens Blumeria graminis Crops Crop 03 medical and health sciences Ascomycota Barley Genetic variation Genetics Grasses Plant breeding Plant Diseases Crop Genetics Host (biology) Organisms Biology and Life Sciences Genetic Variation Hordeum Plant Pathology biology.organism_classification Powdery Mildew Plant Breeding 030104 developmental biology Earth Sciences Crop Science Cereal Crops 010606 plant biology & botany |
| Zdroj: | PLoS ONE, Vol 13, Iss 12, p e0208719 (2018) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Human activities including those in crop gene banks are subject to errors, especially during seed multiplication and maintenance of seed germination. Therefore, the most serious problem of gene banks is authenticity of the accessions and their genotypic purity. There are many methods for determining the identity of varieties, but comparisons between current data and past records are not easy since the latter are often missing. Breeding barley resistant to powdery mildew caused by Blumeria graminis f. sp. hordei (Bgh) was traditionally based on incorporating major genes into new varieties and the results have been published. Our goal was to identify resistance genes to powdery mildew in accessions of the Czech spring barley core collection and compare these data with earlier information to establish the authenticity of the accessions. Two hundred and twenty-three accessions of the collection including 665 single plant progenies were tested. Sixty-four selected reference isolates of Bgh representing the world diversity of the pathogen were used for resistance tests. Twenty-two known resistance genes were postulated either separately or in combinations. In the collection, 151 homogeneous accessions were found, but the resistances of nine of them were inconsistent with published data and in 12 accessions their authenticity is doubtful. The remaining 72 accessions were heterogeneous and comprised 176 resistance genotypes, 54 of which were probably mechanical admixtures of other varieties. There are several pathogens of cereals, e.g. rusts and mildews, against which many resistance genes in host crops have also been exploited. Knowledge of these resistances can assist in maintaining pure and genuine stocks in gene banks. Seed purity and the authenticity of accessions can subsequently be checked with more advanced methods. |
| Databáze: | OpenAIRE |
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