Molecular Mechanism of Cell Membrane Protection by Sugars: A Study of Interfacial H-Bond Networks
| Autor: | Carlos R. Baiz, Euihyun Lee, Cong Xu, Xiao You |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Spectrophotometry Infrared Hydrogen bond Biomolecule Vesicle Water Infrared spectroscopy Hydrogen Bonding Molecular Dynamics Simulation Cell membrane Molecular dynamics Glucose Membrane medicine.anatomical_structure chemistry medicine Biophysics General Materials Science Physical and Theoretical Chemistry Dimyristoylphosphatidylcholine Sugar Unilamellar Liposomes |
| Zdroj: | The Journal of Physical Chemistry Letters. 12:9602-9607 |
| ISSN: | 1948-7185 |
| DOI: | 10.1021/acs.jpclett.1c02451 |
| Popis: | Sugars function as bioprotectants by stabilizing biomolecules during dehydration, thermal stress, and freeze-thaw cycles. A buildup of sugars occurs in many organisms upon their exposure to extreme conditions. Understanding sugar's bioprotective effects on membranes is achieved by characterizing the H-bond networks at the lipid-water interface. Here, we report the headgroup H-bond populations, structures, and dynamics of 1,2-dimyristoyl-sn-glycero-3-phosphocholine vesicles in concentrated glucose solutions using ultrafast two-dimensional infrared spectroscopy in conjunction with molecular dynamics simulations. H-Bond populations and dynamics at the ester carbonyl positions are largely unaffected even at very high, 600 mg/mL, sugar concentrations. In addition, dynamics exhibit a slight nonmonotonic dependence on sugar concentration. Simulations, which are in near-quantitative agreement with measured dynamics, show that the H-bond structure remains largely intact by the existence of sugar. This study shows that the bioprotection of sugar is realized through stable lipid-saccharide-water H-bond networks at the membrane interface that mimic the H-bond networks in pure water. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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