Membrane sidedness of biosynthetic pathways involved in the production of lysophosphatidic acid
| Autor: | Olivier Fourcade, Hugues Chap, Sandra Meijer, François Le Balle, Marie-Françoise Simon |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Blood Platelets
Cancer Research Erythrocytes Phospholipid Mice Inbred Strains In Vitro Techniques Phospholipase Models Biological Phospholipases A Mice chemistry.chemical_compound Phospholipid scrambling Lysophosphatidic acid Genetics Animals Humans Platelet activation Molecular Biology Calcimycin chemistry.chemical_classification Ionophores Cell Membrane Phosphatidic acid Smooth muscle contraction Platelet Activation Cell biology Phospholipases A2 Enzyme chemistry Molecular Medicine lipids (amino acids peptides and proteins) Lysophospholipids biological phenomena cell phenomena and immunity Subcellular Fractions |
| Zdroj: | Advances in Enzyme Regulation. 39:275-284 |
| ISSN: | 0065-2571 |
| DOI: | 10.1016/s0065-2571(98)00024-7 |
| Popis: | Lysophosphatidic acid (LPA) is a novel phospholipid mediator with diverse biological activities such as smooth muscle contraction, and proliferative effects or modifications of cytoskeleton. Activated blood platelets are the best identified source, explaining accumulation of LPA in serum upon blood coagulation. However, the metabolic pathways responsible for LPA synthesis are still poorly known. Using a model of human erythrocytes treated with the calcium ionophore A23187, we have shown that type II secretory phospholipase A2 (sPLA2) is able to produce LPA by hydrolyzing phosphatidic acid exposed on the cell surface after phospholipid scrambling. A similar mechanism does not appear to occur in platelets, where inhibitors of sPLA2 or genetic lack of the enzyme do not modify LPA production. However, this does not definitely eliminate the possibility that LPA is also produced in platelets in the external leaflet of the membrane by other phospholipases, which have to be better characterized. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect