Antigenic Relationship of Brucella and Vibrio Comma
| Autor: | Grace A. Beal, C. W. Eisele, N. B. Mccullough |
|---|---|
| Rok vydání: | 1948 |
| Předmět: | |
| Zdroj: | Journal of Infectious Diseases. 83:55-59 |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1093/infdis/83.1.55 |
| Popis: | rows of the University of Chicago and had been originally obtained from the National Institute of Health?their strain #41. It adhered to the usual description of the species.6 Production of Antiserums.?Young rabbits weighing 4-6 lbs. were employed. All animals were tested prior to immunization and were negative for brucella and cholera agglutinins. Rabbits were infected with brucella by intravenous injection of 1 cc of 24 hr Tryptose broth cultures. Two rabbits were used for each of the three species. For production of cholera -antiserum, standard cholera vaccine* (Inaba and Ogawa strains) was employed. Weekly intravenous injections of 0.25 cc. 0.50 cc and 1.0 cc were given. When satisfactory titres appeared, about three weeks after infecting the rabbits with brucella and two weeks after the last injection of cholera vaccine, the animals were bled from the heart and the serums collected aseptically. After inactivation at 56 C for 30 minutes, they were stored in the refrigerator until used. Technic.?Antigens for the agglutination test were prepared as follows: The 1. Eisele, C. W., McCullough, N. B., Beal, G. A., and Burrows, W. 1946, Proc. Soc. Exper. Biol, and Med. 61: 89. 2. Eisele, C. W., McCullough, N. B., and Beal, G. A. 1948, Ann. Int. Med. 28: 833. 3. Eisele, C. W., McCullough, N. B, Beal, G. A., and Rottschaefer, W. 1947, J.A.M.A. 135: 983. |
| Databáze: | OpenAIRE |
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