Bilirubin inhibits bile acid induced apoptosis in rat hepatocytes
| Autor: | Gregory J. Gores, M. T. Vilei, Chiara Ferraresso, Anna Granato, Maurizio Muraca, R Tolando |
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| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
Liver injury
chemistry.chemical_classification medicine.medical_specialty Reactive oxygen species Antioxidant Bile acid Bilirubin medicine.drug_class medicine.medical_treatment Gastroenterology Biology medicine.disease Free radical scavenger chemistry.chemical_compound Endocrinology Cholestasis chemistry Apoptosis Internal medicine medicine |
| Popis: | Background and aims: Hydrophobic bile acids contribute to hepatocellular injury in cholestasis and rapidly induce apoptosis in vitro; however, unlike Fas agonists, cholestasis does not cause extensive hepatocyte apoptosis. As antioxidants provide protection against bile acid induced liver injury, our premise was that bilirubin, a free radical scavenger with increased plasma levels in the presence of liver disease, could protect hepatocytes against bile acid induced apoptosis. Methods: Freshly isolated rat hepatocytes were incubated for four hours with 100 μmol/l glycochenodeoxycholate (GCDC) alone or with increasing concentrations of unconjugated (UCB) or conjugated (CB) bilirubin. Results: Both UCB and CB inhibited GCDC induced apoptosis in a dose dependent fashion and suppressed the generation of reactive oxygen species by hepatocytes. Conclusions: The antiapoptotic effect of bilirubin associated with its antioxidant properties indicates that hyperbilirubinaemia may have a protective role in liver disease. |
| Databáze: | OpenAIRE |
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