The intron-containing L3 ribosomal protein gene (RPL3): sequence analysis and identification of U43 and of two novel intronic small nucleolar RNAs
| Autor: | Stefano Duga, Massimo Malcovati, Rosanna Asselta, Severino Ronchi, Tatjana Simonic, Maria Luisa Tenchini |
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| Rok vydání: | 2000 |
| Předmět: |
Ribosomal Proteins
Sequence analysis Ribosomal Protein L3 Molecular Sequence Data Biophysics Gene Expression Biology Methylation Biochemistry Cell Line Mice Exon Structural Biology Ribosomal protein Genetics Animals Humans RNA Small Nucleolar Small nucleolar RNA Gene Base Sequence Reverse Transcriptase Polymerase Chain Reaction Intron RNA RNA Probes Introns RNA Ribosomal Cattle Human genome |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1490:225-236 |
| ISSN: | 0167-4781 |
| DOI: | 10.1016/s0167-4781(99)00237-7 |
| Popis: | Isolation and sequencing of bovine and human intron-containing L3 ribosomal protein genes are here reported. They exhibit very similar organisation, both comprising 10 exons and nine introns. A polymorphic locus, involving a 19-bp deletion, was found in intron 6 of the human gene. The frequency of the two alleles has been estimated in 200 haploid genomes. In bovine and human genes intron sequences are rather different, except for limited regions, located in corresponding positions, which show a surprisingly high degree of identity. All these regions contain conserved features defining the box C/D class of small nucleolar RNAs. Demonstration is given that U43 small nucleolar RNA is encoded within the first intron of both bovine and human genes. Single nucleotide sequences, encoding two novel species of small nucleolar RNAs (U82, U83a and U83b), are located in introns 3, 5 and 7. Their expression has been investigated and a possible role of these molecules in 2'-O-ribose methylation of rRNAs is discussed. |
| Databáze: | OpenAIRE |
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