Massive activation of archaeal defense genes during viral infection
| Autor: | Jean-Yves Coppée, Odile Sismeiro, Marleen Voet, Tessa E. F. Quax, John van der Oost, David Prangishvili, Bernd Jagla, Guennadi Sezonov, Marie-Agnès Dillies, Patrick Forterre, Rob Lavigne |
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| Přispěvatelé: | Biologie Moléculaire du Gène chez les Extrêmophiles (BMGE), Institut Pasteur [Paris], Transcriptome et Epigénome (PF2), Institut de génétique et microbiologie [Orsay] (IGM), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Gene Expression Regulation
Viral Immunology Rudiviridae Biology dna Microbiology Virus Host-Parasite Interactions Sulfolobus sulfolobus-solfataricus prokaryotes 03 medical and health sciences Microbiologie Virology Two-Hybrid System Techniques CRISPR [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Gene 030304 developmental biology VLAG toxin-antitoxin systems Regulation of gene expression Genetics host interactions 0303 health sciences 030306 microbiology crispr rnas Archaeal Viruses Sequence Analysis DNA Genome Replication and Regulation of Viral Gene Expression [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Lytic cycle cell-division differential expression analysis turreted icosahedral virus Insect Science Host cell envelope Gene Expression Regulation Archaeal Transcriptome complex |
| Zdroj: | Journal of Virology 87 (2013) 15 Journal of Virology Journal of Virology, American Society for Microbiology, 2013, 87 (15), pp.8419-28. ⟨10.1128/JVI.01020-13⟩ Journal of Virology, 87(15), 8419-8428 Journal of Virology, 2013, 87 (15), pp.8419-28. ⟨10.1128/JVI.01020-13⟩ |
| ISSN: | 0022-538X 1098-5514 |
| DOI: | 10.1128/JVI.01020-13⟩ |
| Popis: | Archaeal viruses display unusually high genetic and morphological diversity. Studies of these viruses proved to be instrumental for the expansion of knowledge on viral diversity and evolution. The Sulfolobus islandicus rod-shaped virus 2 (SIRV2) is a model to study virus-host interactions in Archaea . It is a lytic virus that exploits a unique egress mechanism based on the formation of remarkable pyramidal structures on the host cell envelope. Using whole-transcriptome sequencing, we present here a global map defining host and viral gene expression during the infection cycle of SIRV2 in its hyperthermophilic host S. islandicus LAL14/1. This information was used, in combination with a yeast two-hybrid analysis of SIRV2 protein interactions, to advance current understanding of viral gene functions. As a consequence of SIRV2 infection, transcription of more than one-third of S. islandicus genes was differentially regulated. While expression of genes involved in cell division decreased, those genes playing a role in antiviral defense were activated on a large scale. Expression of genes belonging to toxin-antitoxin and clustered regularly interspaced short palindromic repeat (CRISPR)-Cas systems was specifically pronounced. The observed different degree of activation of various CRISPR-Cas systems highlights the specialized functions they perform. The information on individual gene expression and activation of antiviral defense systems is expected to aid future studies aimed at detailed understanding of the functions and interplay of these systems in vivo . |
| Databáze: | OpenAIRE |
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