Effect of switching glatiramer acetate formulation from 20 mg daily to 40 mg three times weekly on immune function in multiple sclerosis
| Autor: | Yong Lin, Naoko Ito, Kouichi Ito, Suhayl Dhib-Jalbut, Shradha Suresh, Sudhir K. Yadav |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Relapse rate Peripheral blood mononuclear cell Flow cytometry Multiple sclerosis 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Immune system medicine Glatiramer acetate Adverse effect medicine.diagnostic_test business.industry FOXP3 medicine.disease cytokines regulatory T-cells Original Research Paper 030104 developmental biology Immunology glatiramer acetate Neurology (clinical) business 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Multiple Sclerosis Journal-Experimental, Translational and Clinical |
| ISSN: | 2055-2173 |
| Popis: | Background Many RRMS patients who had been treated for over 20 years with GA 20 mg/ml daily (GA20) switched to 40 mg/ml three times-a-week (GA40) to reduce injection-related adverse events. Although GA40 is as effective as GA20 in reducing annualized relapse rate and MRI activity, it remains unknown how switching to GA40 from GA20 affects the development of pathogenic and regulatory immune cells. Objective To investigate the difference in immunological parameters in response to GA20 and GA40 treatments. Methods We analyzed five pro-inflammatory cytokines (IL-1β, IL-23, IL-12, IL-18, TNF-α), and three anti-inflammatory/regulatory cytokines (IL-10, IL-13, and IL-27) in serum. In addition, we analyzed six cytokines (IFN-γ, IL-17A, GM-CSF, IL-10, IL-6, and IL-27) in cultured PBMC supernatants. The development of Th1, Th17, Foxp3 Tregs, M1-like, and M2-like macrophages were examined by flow cytometry. Samples were analyzed before and 12 months post switching to GA40 or GA20. Results Pro- and anti-inflammatory cytokines were comparable between the GA40 and GA20 groups. Development of Th1, Th17, M1-like macrophages, M2-like macrophages, and Foxp3 Tregs was also comparable between the two groups. Conclusions The immunological parameters measured in RRMS patients treated with GA40 three times weekly are largely comparable to those given daily GA20 treatment. |
| Databáze: | OpenAIRE |
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