The Arabidopsis ROP-activated receptor-like cytoplasmic kinase RLCK VI_A3 is involved in control of basal resistance to powdery mildew and trichome branching
| Autor: | Tina Reiner, Attila Fehér, Christina Huesmann, Caroline Hoefle, Ralph Hückelhoven, Dalma Ménesi |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Green Fluorescent Proteins
Mutant Arabidopsis Erysiphe cruciferarum Plant Science Ascomycota Arabidopsis thaliana Kinase activity Disease Resistance Monomeric GTP-Binding Proteins Plant Diseases biology Arabidopsis Proteins Kinase Trichome branching Hordeum Trichomes General Medicine Plants Genetically Modified biology.organism_classification Cell biology Host-Pathogen Interactions Mutation Agronomy and Crop Science Trichome morphogenesis |
| Zdroj: | Plant Cell Reports. 34:457-468 |
| ISSN: | 1432-203X 0721-7714 |
| DOI: | 10.1007/s00299-014-1725-1 |
| Popis: | The Arabidopsis receptor-like cytoplasmic kinase AtRLCK VI_A3 is activated by AtROPs and is involved in trichome branching and pathogen interaction. Receptor-like cytoplasmic kinases (RLCKs) belong to the large superfamily of receptor-like kinases, which are involved in a variety of cellular processes like plant growth, development and immune responses. Recent studies suggest that RLCKs of the VI_A subfamily are possible downstream effectors of the small monomeric G proteins of the plant-specific Rho family, called ‘Rho of plants’ (RAC/ROPs). Here, we describe Arabidopsis thaliana AtRLCK VI_A3 as a molecular interactor of AtROPs. In Arabidopsis epidermal cells, transient co-expression of plasma membrane located constitutively activated (CA) AtROP4 or CA AtROP6 resulting in the recruitment of green fluorescent protein-tagged AtRLCK VI_A3 to the cell periphery. Intrinsic kinase activity of AtRLCK VI_A3 was enhanced in the presence of CA AtROP6 in vitro and further suggested a functional interaction between the proteins. In the interaction of the biotrophic powdery mildew fungus Erysiphe cruciferarum (E. cruciferarum) and its host plant Arabidopsis, Atrlck VI_A3 mutant lines supported enhanced fungal reproduction. Furthermore Atrlck VI_A3 mutant lines showed slightly reduced size and an increase in trichome branch number compared to wild-type plants. In summary, our data suggest a role of the AtROP-regulated AtRLCK VI_A3 in basal resistance to E. cruciferarum as well as in plant growth and cellular differentiation during trichome morphogenesis. Results are discussed in the context of literature suggesting a function of RAC/ROPs in both resistance and susceptibility to pathogen infection. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink