An interspecies signaling system mediated by fusaric acid has parallel effects on antifungal metabolite production by Pseudomonas protegens strain Pf-5 and antibiosis of Fusarium spp
| Autor: | Neal C. Goebel, T. Mark Zabriskie, Marcella D. Henkels, Brenda T. Shaffer, Maria Carolina Quecine, Teresa A. Kidarsa, Joyce E. Loper |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Fusarium Antifungal Agents Transcription Genetic Applied Microbiology and Biotechnology Microbiology 03 medical and health sciences chemistry.chemical_compound Pseudomonas protegens Plant Microbiology Pseudomonas Antibiosis Fusarium oxysporum METABÓLITOS SECUNDÁRIOS Toxoflavin Ecology biology Rhizoxin food and beverages Fusaric Acid biology.organism_classification Culture Media Pyrrolnitrin 030104 developmental biology chemistry Microbial Interactions Metabolic Networks and Pathways Fusaric acid Signal Transduction Food Science Biotechnology |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | Pseudomonas protegens strain Pf-5 is a rhizosphere bacterium that suppresses soilborne plant diseases and produces at least seven different secondary metabolites with antifungal properties. We derived mutants of Pf-5 with single and multiple mutations in biosynthesis genes for seven antifungal metabolites: 2,4-diacetylphoroglucinol (DAPG), pyrrolnitrin, pyoluteorin, hydrogen cyanide, rhizoxin, orfamide A, and toxoflavin. These mutants were tested for inhibition of the pathogens Fusarium verticillioides and Fusarium oxysporum f. sp. pisi . Rhizoxin, pyrrolnitrin, and DAPG were found to be primarily responsible for fungal antagonism by Pf-5. Previously, other workers showed that the mycotoxin fusaric acid, which is produced by many Fusarium species, including F. verticillioides , inhibited the production of DAPG by Pseudomonas spp. In this study, amendment of culture media with fusaric acid decreased DAPG production, increased pyoluteorin production, and had no consistent influence on pyrrolnitrin or orfamide A production by Pf-5. Fusaric acid also altered the transcription of biosynthetic genes, indicating that the mycotoxin influenced antibiotic production by Pf-5 at the transcriptional level. Addition of fusaric acid to the culture medium reduced antibiosis of F. verticillioides by Pf-5 and derivative strains that produce DAPG but had no effect on antibiosis by Pf-5 derivatives that suppressed F. verticillioides due to pyrrolnitrin or rhizoxin production. Our results demonstrated the importance of three compounds, rhizoxin, pyrrolnitrin, and DAPG, in suppression of Fusarium spp. by Pf-5 and confirmed that an interspecies signaling system mediated by fusaric acid had parallel effects on antifungal metabolite production and antibiosis by the bacterial biological control organism. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|