Actin filaments and microtubules regulate endocytosis in marginal cells of the stria vascularis

Autor:	Akinobu Kakigi, Daizo Taguchi, Rie Nishioka, Taizo Takeda, Teruhiko Okada, Masahiko Nishimura
Rok vydání:	2008
Předmět:	health care facilities manpower and services Guinea Pigs education Endosomes macromolecular substances Endocytosis Microtubules Clathrin Bulk endocytosis Protein filament Microtubule Animals health care economics and organizations Actin biology Cell Membrane Stria Vascularis Biological Transport General Medicine biology.organism_classification Actina Cell biology Actin Cytoskeleton Tubulin Peroxidases Otorhinolaryngology Ferritins biology.protein Female
Zdroj:	Acta Oto-Laryngologica. 128:856-860
ISSN:	1651-2251 0001-6489
Popis:	Cationized ferritin (CF) was internalized via clathrin-mediated endocytosis. This process depends on clathrin, actin filaments, and microtubules. Microperoxidase (MPO) was internalized via a clathrin- and caveolin-independent endocytic pathway, which was partially dependent on microtubules but independent of clathrin and actin filaments.We investigated the role of actin filaments and microtubules in the transport of endocytic carrier vesicles (ECVs) from the plasma membrane to the early sorting endosomes, using CF and MPO as tracers.Fifty-five guinea pigs were used. The animals were divided into a CF endocytosis group and an MPO endocytosis group. These groups consisted of control, nocodazole-treated, cytochalasin (Cyt D)-treated, Cyt D + nocodazole-treated, and geldanamycin-treated subgroups.For CF endocytosis, the following results were obtained. In the nocodazole experiment, in which microtubules were disrupted to form monomeric tubulin, the number of ECVs loaded with CF was greatly decreased. In the Cyt D experiment, in which the actin filaments were disrupted to form monomers, the number of ECVs labeled with CF was also greatly decreased. In the geldanamycin experiment, in which clathrin-mediated endocytosis was regulated and actin stress fibers were dissolved, the endocytosis of CF was severely inhibited. For MPO endocytosis, in the nocodazole experiment, the endocytosis of MPO was markedly suppressed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5e982c7ba1716750a34372abca47fa33 https://doi.org/10.1080/00016480701777373 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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