Mesenchymal stem cells combined with an artificial dermal substitute improve repair in full-thickness skin wounds
| Autor: | Paloma Dias da Cruz, Dilmar Leonardi, Rosalva Thereza Meurer, Daniel Oberdoerfer, Roberto C. Chem, Marilda da Cruz Fernandes, Gustavo A. Pereira-Filho, Melissa Camassola, Nance Beyer Nardi, Marcelo Vargas |
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| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
Cell Culture Techniques Clinical uses of mesenchymal stem cells Cell Count Mice Transgenic Mesenchymal Stem Cell Transplantation Critical Care and Intensive Care Medicine Transplantation Autologous Mice Animals Medicine Bone Marrow Transplantation Stem cell transplantation for articular cartilage repair Skin Artificial Wound Healing integumentary system business.industry Mesenchymal stem cell General Medicine Combined Modality Therapy Surgery Transplantation Disease Models Animal medicine.anatomical_structure Emergency Medicine Cancer research Wounds and Injuries Bone marrow Stem cell business Wound healing Whole Bone Marrow |
| Zdroj: | Burns. 38:1143-1150 |
| ISSN: | 0305-4179 |
| Popis: | Autografts represent the gold standard for the treatment of full thickness burns. Factors such as lack of suitable donor sites and poor skin quality, however, have led to the development of artificial dermal substitutes. The investigation of mechanisms leading to enhanced functionality of these skin substitutes has been attracting great attention. This study aimed to investigate the effect of autologous stem cells on the integration and vascularization of a dermal substitute in full-thickness skin wounds, in a murine model. Two cell populations were compared, whole bone marrow cells and cultivated mesenchymal stem cells, isolated from mice transgenic for the enhanced green fluorescent protein, which allowed tracking of the transplanted cells. The number of cells colonizing the dermal substitute, as well as vascular density, were higher in mice receiving total bone marrow and particularly mesenchymal stem cells, than in control animals. The effect was more pronounced in animals treated with mesenchymal stem cells, which located primarily in the wound bed, suggesting a paracrine therapeutic mechanism. These results indicate that combining mesenchymal stem cells with artificial dermal substitutes may represent an important potential modality for treating full thickness burns, even in allogeneic combinations due to the immunoregulatory property of these cells. |
| Databáze: | OpenAIRE |
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