N-acetylcysteine protects against bupivacaine-induced myotoxicity caused by oxidative and sarcoplasmic reticulum stress in human skeletal myotubes
| Autor: | Annick Bourret, Guillaume Py, Fabien Pillard, Karine Nouette-Gaulain, Jacques Mercier, Olivier Galbes, Stefan Matecki, Alexandre Philips, Xavier Capdevila |
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| Přispěvatelé: | Différenciation Cellulaire et Croissance (DCC), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2), Muscle et pathologies, Université Montpellier 1 (UM1)-IFR3, Université Montpellier 1 (UM1)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM), Physiopathologie mitochondriale, Université Bordeaux Segalen - Bordeaux 2-Institut National de la Santé et de la Recherche Médicale (INSERM), Service d'exploration de la fonction respiratoire et de médecine du sport, CHU Toulouse [Toulouse], Service de physiologie clinique, Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Hôpital Arnaud de Villeneuve, Service d'anesthésie et de réanimation A, Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), INSERM ERI 25, F-34295 Montpellier, France, Université Montpellier 1, Montpellier, France, La Région Languedoc-Roussillon, Montpellier, France, L'Association Française contre les Myopathies, Paris, France, Physiologie & médecine expérimentale du Cœur et des Muscles [U 1046] (PhyMedExp), Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Philips, Alexandre, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse) |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Sarcoplasm
Muscle Fibers Skeletal douleurs musculaires Apoptosis [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] Pharmacology medicine.disease_cause Antioxidants anesthésiques locaux 0302 clinical medicine 030202 anesthesiology Myocyte Cells Cultured chemistry.chemical_classification 0303 health sciences MESH: Muscle Skeletal MESH: Muscle Fibers Skeletal MESH: Oxidative Stress Myogenesis MESH: Reactive Oxygen Species Bupivacaine 3. Good health Sarcoplasmic Reticulum medicine.anatomical_structure Anesthesia douleurs d'origine iatrogène MESH: Bupivacaine MESH: Sarcoplasmic Reticulum MESH: Cells Cultured 03 medical and health sciences MESH: Acetylcysteine cellules musculaires squelettiques humaines medicine [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] Humans Muscle Skeletal 030304 developmental biology Reactive oxygen species MESH: Humans business.industry Endoplasmic reticulum MESH: Apoptosis MESH: Antioxidants Skeletal muscle effet protecteur de la N-acétylcystéine Acetylcysteine myotoxicité Oxidative Stress Anesthesiology and Pain Medicine chemistry business Reactive Oxygen Species Oxidative stress |
| Zdroj: | Anesthesiology Anesthesiology, Lippincott, Williams & Wilkins, 2010, 113 (3), pp.560-9. ⟨10.1097/ALN.0b013e3181e4f4ec⟩ Anesthesiology, 2010, 113 (3), pp.560-9. ⟨10.1097/ALN.0b013e3181e4f4ec⟩ |
| ISSN: | 0003-3022 |
| Popis: | Background Local anesthetics offer the benefits of extended analgesia with greater patient satisfaction and faster rehabilitation compared with intravenous morphine. These benefits, however, can be offset by adverse iatrogenic muscle pain. Here, the authors investigate the mechanisms of local anesthetic-induced myotoxicity and assess the protective effect of N-acetylcysteine. Methods The authors used primary cell cultures of human skeletal muscle myoblasts to study local anesthetic adverse effects. Production of reactive oxygen species was investigated in human skeletal myotubes by fluorescence microscopy. Expression of sarcoplasmic/endoplasmic reticulum stress markers and induction of apoptosis were followed by immunofluorescence and Western blot analysis. Finally, the effect of N-acetylcysteine on bupivacaine-induced myotoxicity was investigated in vitro. Results Bupivacaine sequentially induced reactive oxygen species production, oxidative stress, sarcoplasmic/endoplasmic reticulum stress, and activation of caspases 9 and 7 in human differentiated myoblasts. These iatrogenic effects were prevented by N-acetylcysteine. Conclusions The authors demonstrated a protective effect of N-acetylcysteine against bupivacaine-induced sarcoplasmic/endoplasmic reticulum stress and apoptosis in primary human skeletal muscle cell. |
| Databáze: | OpenAIRE |
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