Calcium-Independent Activation of Extracellular Signal-Regulated Kinases 1 and 2 by Cyclic Strain
| Autor: | Teiji Takei, Ira Mills, Masataka Ikeda, Bauer E. Sumpio |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Intracellular Fluid
Biophysics chemistry.chemical_element Gadolinium Biology Calcium Biochemistry chemistry.chemical_compound Extracellular Animals Channel blocker Phosphorylation Egtazic Acid Molecular Biology Cells Cultured Chelating Agents Mitogen-Activated Protein Kinase 1 Calcium metabolism Mitogen-Activated Protein Kinase 3 Kinase Cell Biology Calcium Channel Blockers Molecular biology Hydroquinones Cell biology Enzyme Activation EGTA chemistry Calcium-Calmodulin-Dependent Protein Kinases Cattle Endothelium Vascular Stress Mechanical Mitogen-Activated Protein Kinases Signal transduction Extracellular Space |
| Zdroj: | Biochemical and Biophysical Research Communications. 247:462-465 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1998.8811 |
| Popis: | We have previously demonstrated that cyclic strain induces extracellular signal-regulated kinases 1 and 2 (ERK1/2) activation in endothelial cells (EC). The aim of this study was to investigate the effect of Ca2+ on the activation of ERK1/2. Bovine aortic EC were pretreated with a chelator of extracellular Ca2+, ethylaneglycol-bis(aminoethylether)-tetra-acetate (EGTA), a depleter of Ca2+ pools, 2,5-Di-(tert-butyl)-1,4-benzohydroquinone (BHQ), or a Ca2+ channel blocker, GdCl3, and subjected to an average 10 % strain at a rate of 60 cycles/min for 10 min. BHQ and GdCl3 did not inhibit the strain-induced ERK1/2 activation. Chelation of normal extracellular Ca2+ (1.8 mM) medium with EGTA (3 mM) acutely stimulated baseline phosphorylation and activation of ERK1/2, thereby obscuring any strain-induced activation of ERK1/2. However, in EC preincubated for 24 hours in Ca2+-free medium, elevated baseline phosphorylation was minimally activated by EGTA (200 microM) such that cyclic strain stimulated ERK1/2 in the presence or absence of BHQ. These results suggest a Ca2+ independence of the ERK1/2 signaling pathway by cyclic strain. Copyright 1998 Academic Press. |
| Databáze: | OpenAIRE |
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