Indirubin inhibits cell proliferation, migration, invasion and angiogenesis in tumor-derived endothelial cells
Autor: | Yu Chen, Lin Qian, Baiping An, Lan Lan, Chaofu Zhu, Zhuohong Li, Xiuyun He, Shijie Li |
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Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Angiogenesis proliferation migration lcsh:RC254-282 OncoTargets and Therapy Umbilical vein indirubin 03 medical and health sciences chemistry.chemical_compound angiogenesis 0302 clinical medicine Pharmacology (medical) Original Research Tube formation Migration invasion Cell growth Chemistry other Tumor-Derived lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens invasion In vitro Td-EC 030104 developmental biology Oncology Cell culture 030220 oncology & carcinogenesis Cancer research Indirubin Wound healing |
Zdroj: | OncoTargets and therapy OncoTargets and Therapy, Vol Volume 11, Pp 2937-2944 (2018) |
ISSN: | 1178-6930 |
Popis: | Zhuohong Li, Chaofu Zhu, Baiping An, Yu Chen, Xiuyun He, Lin Qian, Lan Lan, Shijie Li Department of Oncology, The Affiliated Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China Purpose: Hepatocellular carcinoma is one of the most predominant malignancies with high fatality rate and its incidence is rising at an alarming rate because of its resistance to radio- and chemotherapy. Indirubin is the major active anti-tumor ingredient of a traditional Chinese herbal medicine. The present study aimed to analyze the effects of indirubin on cell proliferation, migration, invasion, and angiogenesis of tumor-derived endothelial cells (Td-EC). Methods: Td-EC were derived from human umbilical vein endothelial cells (HUVEC) by treating HUVEC with the conditioned medium of human liver cancer cell line HepG2. Cell proliferation, migration, invasion, and angiogenesis were assessed by MTT, wound healing, in vitro cell invasion, and in vitro tube formation assay. Results: Td-EC were successfully obtained from HUVEC cultured with 50% culture supernatant from serum-starved HepG2 cells. Indirubin significantly inhibited Td-EC proliferation in a dose- and time-dependent manner. Indirubin also inhibited Td-EC migration, invasion, and angiogenesis. However, indirubin’s effects were weaker on HUVEC than Td-EC. Conclusion: Indirubin significantly inhibited Td-EC proliferation, migration, invasion, and angiogenesis. Keywords: indirubin, Td-EC, proliferation, migration, invasion, angiogenesis |
Databáze: | OpenAIRE |
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