Bovine herpesviruses induce different cell death forms in neuronal and glial-derived tumor cell cultures
| Autor: | Eduardo Furtado Flores, Helena Lage Ferreira, Ana Carolina G. Rosa, Bruna R. S. M. Oliveira, Flávia Vieira, Lucas Hidenori Okamura, Camila Silva-Frade, Roberto Gameiro, Tereza C. Cardoso |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Universidade de São Paulo (USP), Universidade Federal de Sergipe (UFS) |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Programmed cell death Necroptosis Gene Expression Oncolytic viruses Apoptosis Pathogenesis Biology Virus Replication Virus Animal herpesvirus Mice Necrosis 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Cell Line Tumor Virology Animals Humans Antigens Viral Herpesvirus 1 Bovine Neurons Herpesvirus 5 Bovine Tumor Necrosis Factor-alpha biology.organism_classification Mitochondria Rats Oncolytic virus Oncolytic Viruses Oxidative Stress 030104 developmental biology Bovine herpesvirus 5 Neurology Viral replication Organ Specificity Oxidative stress Cell culture Host-Pathogen Interactions Cattle PATOGENIA ANIMAL Neurology (clinical) Reactive Oxygen Species Neuroglia 030217 neurology & neurosurgery |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1538-2443 1355-0284 |
| Popis: | Made available in DSpace on 2018-11-26T15:37:32Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-12-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Oncolytic viruses have the ability to infect tumor cells and leave healthy cells intact. In this study, bovine herpesvirus 1 (BHV1; Los Angeles, Cooper, and SV56/90 strains) and bovine herpesvirus 5 (BHV5; SV507/99 and GU9457818 strains) were used to infect two neuronal tumor cell lineages: neuro2a (mouse neuroblastoma cells) and C6 (rat glial cells). BHV1 and BHV5 strains infected both cell lines and positively correlated with viral antigen detection (p < 0.005). When neuro2a cells were infected by Los Angeles, SV507/99, and GU9457818 strains, 40 % of infected cells were under early apoptosis and necroptosis pathways. Infected C6 cells were > 40 % in necroptosis phase when infected by BHV5 (GU9457818 strain). Blocking caspase activation did not interfere with cell death. However, when necroptosis was blocked, 60-80 % of both infected cells with either virus switched to early apoptosis pathway with no interference with virus replication. Moreover, reactive oxygen species production and mitochondrial membrane dysfunction were detected at high levels in both infected cell lines. In spite of apoptosis and necroptosis blockage, tumor necrosis factor alpha (TNFA) and virus transcription were positively correlated for all viral strains studied. Thus, these results contribute to the characterization of BHV1 and BHV5 as potential oncolytic viruses for non-human cells. Nonetheless, the mechanisms underlying their oncolytic activity in human cells are still to be determined. Univ Estadual Paulista, DAPSA Dept, Lab Anim Virol & Cell Culture, BR-16050680 Sao Paulo, Brazil FZEA USP, Dept Med Vet, Ave Duque Caxias Norte 225, BR-13635900 Pirassununga, SP, Brazil Univ Fed Santa Maria, Virol Sect, BR-97115900 Santa Maria, RS, Brazil Univ Estadual Paulista, DAPSA Dept, Lab Anim Virol & Cell Culture, BR-16050680 Sao Paulo, Brazil FAPESP: 2012/16715-4 CNPq: 500063/2014-1 |
| Databáze: | OpenAIRE |
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