Spectroscopic studies of alpha tocopherol interaction with a model liposome and its influence on oxidation dynamics
Autor: | Marin Kosović, Dubravka Krilov, Kristina Serec |
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Rok vydání: | 2014 |
Předmět: |
alpha-Tocopherol
chemistry.chemical_element Photochemistry Oxygen Antioxidants Analytical Chemistry Ion law.invention chemistry.chemical_compound law Spectroscopy Fourier Transform Infrared liposome α – tocopherol lipid layer oxidation FT-IR EPR heterocyclic compounds Lipoprotein oxidation Fourier transform infrared spectroscopy Electron paramagnetic resonance Lipid bilayer Instrumentation Spectroscopy Liposome Chromatography food and beverages Oxidants Atomic and Molecular Physics and Optics chemistry Liposomes lipids (amino acids peptides and proteins) Oxidation-Reduction |
Zdroj: | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 129:588-593 |
ISSN: | 1386-1425 |
Popis: | The influence of α – tocopherol on the surface conformation of liposome, as a model component of lipoproteins, and its role in oxidation process were studied. FT-IR spectra from suspensions of neat liposome, mixtures of liposome and α – tocopherol and liposome with incorporated α – tocopherol were analyzed. When α – tocopherol was incorporated into liposome, intensities of some bands were decreased or increased in comparison with the spectra of liposome and α – tocopherol mixture. These changes reflect the different localization of α – tocopherol in two types of liposome suspensions. The oxidation of liposome suspensions was initiated by addition of cupric ions. After prolonged oxidation, the differences in FT-IR spectra of oxidized samples were recorded. Differences were observed in comparison with spectra of native and oxidized liposomes were analyzed. The rate of oxidation was measured by EPR oximetry. Oxidation was generally very slow, but faster in liposome without α – tocopherol, indicating the protective role of α – tocopherol against liposome oxidation. On the other hand, liposome suspensions with EDTA in the buffer were not oxidized at all, while those with α – tocopherol and liposome mixture were only slightly oxidized. In this case the consumption of oxygen was the result of liposome oxidation supported by α – tocopherol. These results reflect the ambivalent role of α – tocopherol in liposome oxidation, similarly to findings in studies of lipoprotein oxidation. |
Databáze: | OpenAIRE |
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