Affinity-free enrichment and mass spectrometry analysis of the ovarian cancer biomarker CA125 (MUC16) from patient-derived ascites
| Autor: | Roberta Fritz-Klaus, Mark R. Etzel, Rebecca J. Whelan, Saahil Javeri, Manish S. Patankar, Niharika Patankar, Naviya Schuster-Little |
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| Rok vydání: | 2021 |
| Předmět: |
endocrine system diseases
Peptide Proteomics Mass spectrometry Biochemistry Mass Spectrometry Article Analytical Chemistry 03 medical and health sciences 0302 clinical medicine Tandem repeat Biomarkers Tumor Electrochemistry medicine Humans Environmental Chemistry Spectroscopy 030304 developmental biology Ovarian Neoplasms chemistry.chemical_classification 0303 health sciences medicine.diagnostic_test biology Chemistry Ascites Membrane Proteins medicine.disease female genital diseases and pregnancy complications CA-125 Antigen 030220 oncology & carcinogenesis Immunoassay biology.protein Biomarker (medicine) Female Antibody Ovarian cancer |
| Zdroj: | Analyst |
| ISSN: | 1364-5528 0003-2654 |
| DOI: | 10.1039/d0an01701a |
| Popis: | Developing a mass spectrometry-based assay for the ovarian cancer biomarker CA125 (MUC16) is a desirable goal, because it may enable detection of molecular regions that are not recognized by antibodies and are therefore analytically silent in the current immunoassay. Additionally, the ability to characterize the CA125 proteoforms expressed by individuals may offer clinical insight. Enrichment of CA125 from malignant ascites may provide a high-quality source of this important ovarian cancer biomarker, but a reliable strategy for such enrichment is currently lacking. Beginning with crude ascites isolated from three individual patients with high grade serous ovarian cancer, we enriched for MUC16 using filtration, ion exchange, and size exclusion chromatography and then performed bottom-up proteomics on the isolated proteins. This approach of enrichment and analysis reveals that the peptides detected via mass spectrometry map to the SEA domain and C-loop regions within the tandem repeat domains of CA125 and that peptide abundance correlates with clinical CA125 counts. |
| Databáze: | OpenAIRE |
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