Distribution and Specific Identification of Papillomavirus Major Capsid Protein Epitopes by Immunocytochemistry and Epitope Scanning of Synthetic Peptides
| Autor: | A. Bennett Jenson, X. W. Jin, Lex M. Cowsert, Philip S. Lim, John P. Sundberg, Lai Y. Lim, Y. Nakai |
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| Rok vydání: | 1990 |
| Předmět: |
viruses
Fibroma Epitope Epitopes Capsid Animals Immunology and Allergy Antigens Viral Papillomaviridae Bovine papillomavirus Antiserum Papilloma biology Linear epitope Immune Sera Antibodies Monoclonal biochemical phenomena metabolism and nutrition biology.organism_classification Immunohistochemistry Virology Molecular biology Tumor Virus Infections Infectious Diseases Polyclonal antibodies biology.protein Reagent Kits Diagnostic Antibody |
| Zdroj: | Journal of Infectious Diseases. 162:1263-1269 |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1093/infdis/162.6.1263 |
| Popis: | Monoclonal (MAbs) and polyclonal antibodies were produced against the major capsid protein of detergent-disrupted, purified bovine papillomavirus type 1 (BPV-1). The precise locations of the corresponding epitopes were identified by the reactivity of MAbs and selected polyclonal antibodies with synthetic, overlapping, hexameric peptides corresponding with 95% of the BPV-1 major capsid protein. The topography of these epitopes was determined by reactivity of antibodies with intact (conformational and nonconformational surface epitopes) and disrupted (external or internal nonconformational epitopes) BPV-1 virions. The distribution of epitopes in various papillomaviruses of 13 different species was determined by reactivity of the MAbs and polyclonal sera with productively infected, formalin-fixed papillomas, fibropapillomas, and fibromas. Epitope scanning, using MAbs and polyclonal antisera, resulted in the precise location of BPV-1 hexameric epitopes that could be correlated with their topography on the capsid and distribution in papillomatous lesions of various species. |
| Databáze: | OpenAIRE |
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