The Resistance and Integrase Genes of pACM1, a Conjugative Multiple-Resistance Plasmid, fromKlebsiella oxytoca
| Autor: | Richard A. Venezia, Marilyn A. Kacica, Ronald J. Limberger, Karen E. Preston, Wendy A. Archinal |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
DNA
Bacterial Tetracycline R Factors Molecular Sequence Data Restriction Mapping Biology Integron Microbiology Restriction map Plasmid Klebsiella medicine Humans Amino Acid Sequence Cloning Molecular Molecular Biology Gene Genetics Base Sequence Integrases Klebsiella oxytoca biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Drug Resistance Multiple Integrase Gene cassette Genes Bacterial Conjugation Genetic biology.protein bacteria Genes MDR medicine.drug |
| Zdroj: | Plasmid. 37:105-118 |
| ISSN: | 0147-619X |
| Popis: | pACM1 is an 85-kb conjugative plasmid from a clinical isolate of Klebsiella oxytoca that encodes resistance to β-lactams (mediated by SHV-5 extended spectrum β-lactamase), trimethoprim, sulfonamides, tetracycline, aminoglycosides, and mercuric chloride. The expression of the aminoglycoside resistance is difficult to detect, which could have clinical implications. A region of pACM1 containing five resistance genes and two putative integrons was characterized by restriction mapping and partial DNA sequencing. One integron appears to be class I ( sulI type); the second lacks a recognizable 3′ conserved segment. Neither integron has the Bam HI site predicted for the 5′ conserved segment. Plasmids encoding SHV-5 from other bacterial strains appear to be closely related to pACM1 by restriction enzyme analysis, but have resistance/integron regions that vary in size and content from that of pACM1. Integrase-mediated recombination might be responsible for genetic divergence in a widely distributed family of pACM1-like plasmids. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect