The Effect of Bioindicator Preparation and Storage on Thermal Resistance of Bacillus stearothermophilus Spores

Autor: Marina Ishii, Irene Alexeevna Machoshvili, Marcelo Rodrigues Marques, Thereza Christina Vessoni Penna
Rok vydání: 2002
Předmět:
Zdroj: Applied Biochemistry and Biotechnology. :525-538
ISSN: 0273-2289
Popis: Paper strips inoculated with spores of Bacillus stearothermophilus ATCC 7953 were conventionally dried (lot 1) and lyophilized (lot 2); stored in defined environments of 32 and 86% relative humidity at 10, 25 and 33 degrees C for 210 d; and submitted to moist heat treatments at 121 degrees C. A significant decrease in thermal resistance from initial starting levels was found for lyophilized bioindicators stored at 86% relative humidity. The respective average D121 degrees C values were 1.55+/-0.05 and 1.37+/-0.10 min for lyophilized bioindicators stored at 32 and 86% relative humidity; and 1.65+/-0.15 min and 1.57+/-0.11 min for dried bioindicators stored in the same environments.
Databáze: OpenAIRE