The Effect of Bioindicator Preparation and Storage on Thermal Resistance of Bacillus stearothermophilus Spores
Autor: | Marina Ishii, Irene Alexeevna Machoshvili, Marcelo Rodrigues Marques, Thereza Christina Vessoni Penna |
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Rok vydání: | 2002 |
Předmět: |
Spores
Bacterial Analysis of Variance Bacteriological Techniques Hot Temperature biology Thermal resistance Bioengineering General Medicine Sterilization (microbiology) biology.organism_classification Applied Microbiology and Biotechnology Biochemistry Bacillales Spore Microbiology Geobacillus stearothermophilus Regression Analysis Relative humidity Food science D-value Molecular Biology Bioindicator Biotechnology |
Zdroj: | Applied Biochemistry and Biotechnology. :525-538 |
ISSN: | 0273-2289 |
Popis: | Paper strips inoculated with spores of Bacillus stearothermophilus ATCC 7953 were conventionally dried (lot 1) and lyophilized (lot 2); stored in defined environments of 32 and 86% relative humidity at 10, 25 and 33 degrees C for 210 d; and submitted to moist heat treatments at 121 degrees C. A significant decrease in thermal resistance from initial starting levels was found for lyophilized bioindicators stored at 86% relative humidity. The respective average D121 degrees C values were 1.55+/-0.05 and 1.37+/-0.10 min for lyophilized bioindicators stored at 32 and 86% relative humidity; and 1.65+/-0.15 min and 1.57+/-0.11 min for dried bioindicators stored in the same environments. |
Databáze: | OpenAIRE |
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