Scientific Validation of Ethnomedicinal Use ofIpomoea batatasL. Lam. as Aphrodisiac and Gonadoprotective Agent against Bisphenol A Induced Testicular Toxicity in Male Sprague Dawley Rats

Autor: Fatima Ijaz, Muhammad Waleed Baig, Muhammad Rashid Khan, Bakht Nasir, Ihsan-ul Haq, Muhammad Majid
Rok vydání: 2019
Předmět:
Male
0301 basic medicine
Article Subject
Phytochemicals
lcsh:Medicine
Antioxidants
General Biochemistry
Genetics and Molecular Biology
Rats
Sprague-Dawley
Sexual Behavior
Animal
03 medical and health sciences
Rutin
chemistry.chemical_compound
Follicle-stimulating hormone
0302 clinical medicine
Phenols
Testis
Caffeic acid
Animals
Pakistan
Aphrodisiac
Benzhydryl Compounds
Ipomoea batatas
Chromatography
High Pressure Liquid
Flavonoids
Behavior
Animal
General Immunology and Microbiology
Traditional medicine
Plant Extracts
Aphrodisiacs
lcsh:R
General Medicine
Rats
Semen Analysis
Plant Tubers
Sexual Dysfunction
Physiological
030104 developmental biology
chemistry
Phytochemical
030220 oncology & carcinogenesis
Models
Animal
Toxicity
Female
Myricetin
Medicine
Traditional
Luteinizing hormone
Gonadal Hormones
Research Article
Zdroj: BioMed Research International, Vol 2019 (2019)
BioMed Research International
ISSN: 2314-6141
2314-6133
DOI: 10.1155/2019/8939854
Popis: Sweet potato (Ipomoea batatasL. Lam.), known as “Shakarqandi” in Pakistan, is an imperative root vegetable with large size, traditionally used as aphrodisiac, antiprostatic, anti-inflammatory, antidiabetic, cardiotonic, and anticancer agent. Present study was conducted to gauge aphrodisiac potential ofIpomoea batatasethyl acetate (IPT-EA, IPA-EA) and methanol (IPT-M, IPA-M) extracts from tuber and aerial part, respectively, via behavioral and biochemical tests and their possible protective role in BPA-induced gonadotoxicity at the dose 300 mg/kg in male Sprague Dawley rats. Phytochemical analysis was done qualitatively and quantitatively through total phenolic and flavonoid content (TPC and TFC) and high performance liquid chromatographic (HPLC-DAD) fingerprinting while antioxidant profiling used multimodein vitroassays. To calculate sexual excitement mount latency, intromission latency, mount frequency, intromission frequency, ejaculatory latency, and postejaculatory interval were examined while for biochemical ratification semen characteristics, levels of testosterone, follicle stimulating hormone (FSH), luteinizing hormone (LH), and estradiol were measured. Gonadoprotective ability was assessed through comet assay and histomorphological examination of testes. Qualitative analysis ensured the presence of phenols, flavonoids, tannins, anthocyanin, saponins, coumarins, terpenoids, and betacyanin. Quantitatively maximal TPC (304.32±7.20 μg GAE/mg dry extract) and TFC (214.77±4.09 μg QE/mg DE) were estimated in IPA-EA extract. IPT-EA yielded maximum rutin (7.3±0.12) and myricetin (2.7±0.14 μg/mg DE) while IPA-EA and IPA-M yielded maximum caffeic acid (4.05±0.22 and 1.92±0.17 μg/mg DE, respectively) in HPLC-DAD analysis. Extracts enhanced sexual excitement, improved semen quality, levels of testosterone, FSH, LH, and estradiol, and successfully attenuated toxic effects of BPA. Levels of endogenous antioxidant enzymes (CAT, SOD, POD, and GSH) were restored and NO abundance was minimized. Significant stimulation in sexual behavior, amelioration of toxicity symptoms, elevated spermatic production, raised viability, vitalized levels of gonadal hormones, maintained endogenous enzymes, genoprotection, and reformed testicular histology endorsedI. batatasas a better aphrodisiac alternative and gonadoprotective agent.
Databáze: OpenAIRE
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